Journal article
Comparison of Trichoplusia ni BTI‐Tn‐5B1‐4 (high five™) and Spodoptera frugiperda Sf‐9 insect cell line metabolism in suspension cultures
Biotechnology and bioengineering, Vol.55(6), pp.909-920
09/20/1997
DOI: 10.1002/(SICI)1097-0290(19970920)55:6<909::AID-BIT8>3.0.CO;2-K
PMID: 18636600
Abstract
Nutrient utilization and byproduct accumulation were monitored in Spodoptera frugiperda Sf‐9 and Trichoplusia ni BTI‐Tn‐5B1‐4 (High Five™) cell lines during growth and following viral infection in suspension cultures in order to develop a better understanding of cell metabolism and to acquire information relevant to large scale fed‐batch bioreactors. The utilization of glucose, dissolved oxygen, and amino acids were monitored in Sf‐9 cell cultures grown in Sf‐900 II serum‐free medium (SFM) and in High Five™ cell cultures grown in both Sf‐900 II and Express Five SFM. Using the optimal medium for each cell line, i.e., Sf‐900 II SFM for Sf‐9 cells and Express Five SFM for High Five™ cells, the cell growth rate, maximum cell density, specific glucose and glutamine utilization rates, and specific alanine production rate were comparable during cell growth. In addition, the expression level of recombinant human tissue plasminogen activator was comparable in the two cell lines on a per cell basis. It was found, however, that lactate and ammonia accumulated in High Five™ cell cultures, but not in Sf‐9 cell cultures. In addition, High Five™ cells utilized asparagine more rapidly than glutamine, whereas Sf‐9 cells consumed only minimal asparagine, and the oxygen utilization rate was significantly higher in High Five™ cell cultures. It was also found that the medium had a significant effect on High Five™ cell metabolism, e.g., the specific glucose utilization rate and the specific lactate and alanine production rates were significantly higher in Sf‐900 II SFM than in Express Five SFM. In addition, the maximum cell density and specific asparagine utilization rate were significantly higher in Express Five SFM. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55:909–920, 1997.
Details
- Title: Subtitle
- Comparison of Trichoplusia ni BTI‐Tn‐5B1‐4 (high five™) and Spodoptera frugiperda Sf‐9 insect cell line metabolism in suspension cultures
- Creators
- Martin RhielChristine M Mitchell‐LogeanDavid W Murhammer
- Resource Type
- Journal article
- Publication Details
- Biotechnology and bioengineering, Vol.55(6), pp.909-920
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- DOI
- 10.1002/(SICI)1097-0290(19970920)55:6<909::AID-BIT8>3.0.CO;2-K
- PMID
- 18636600
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- Number of pages
- 12
- Grant note
- National Science Foundation (BES‐9318670)
- Language
- English
- Date published
- 09/20/1997
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003452002771
Metrics
29 Record Views