Journal article
Comparison of histochemical methods for murine eosinophil detection in a RSV vaccine-enhanced inflammation model
Toxicologic pathology, Vol.37(2), pp.249-255
2009
DOI: 10.1177/0192623308329342
PMCID: PMC2664312
PMID: 19181630
Abstract
A comparative study of histochemical detection of eosinophils in fixed murine tissue is lacking. Five histochemical methods previously reported for eosinophil detection were quantitatively and qualitatively compared in an established murine RSV vaccine-enhanced inflammation model. Nonspecific neutrophil staining was evaluated in tissue sections of neutrophilic soft tissue lesions and bone marrow from respective animals. Eosinophils had granular red to orange-red cytoplasmic staining, depending on the method, whereas neutrophils had, when stained, a more homogenous cytoplasmic pattern. Nonspecific background staining of similar coloration was variably seen in arterial walls and erythrocytes. Astra Blue/Vital New Red, Congo Red, Luna, Modified Hematoxylin & Eosin, and Sirius Red techniques were all effective in detecting increased eosinophil recruitment compared to controls; however, differences in eosinophil quantification significantly varied between techniques. Astra Blue/Vital New Red had the best specificity for differentiating eosinophils and neutrophils, but had a reduced ability to enumerate eosinophils and was the most time intensive. The Luna stain had excessive non specific staining of tissues and a reduced enumeration of infiltrating eosinophils making it suboptimal. For multiple parameters such as eosinophil detection, specificity, and contrast with background tissues, the Sirius Red followed by Congo Red and Modified Hematoxylin & Eosin methods were useful, each with their own staining qualities.
Details
- Title: Subtitle
- Comparison of histochemical methods for murine eosinophil detection in a RSV vaccine-enhanced inflammation model
- Creators
- David K Meyerholz - Departments of Pathology (DKM, MAG) and Microbiology (SMV), Roy J. and Lucille A. Carver College of Medicine; and Interdisciplinary Graduate Program in Immunology (EMC, SMV); University of Iowa, Iowa City, Iowa, 52242Michelle A Griffin - Departments of Pathology (DKM, MAG) and Microbiology (SMV), Roy J. and Lucille A. Carver College of Medicine; and Interdisciplinary Graduate Program in Immunology (EMC, SMV); University of Iowa, Iowa City, Iowa, 52242Elaine M Castilow - Departments of Pathology (DKM, MAG) and Microbiology (SMV), Roy J. and Lucille A. Carver College of Medicine; and Interdisciplinary Graduate Program in Immunology (EMC, SMV); University of Iowa, Iowa City, Iowa, 52242Steven M Varga - Departments of Pathology (DKM, MAG) and Microbiology (SMV), Roy J. and Lucille A. Carver College of Medicine; and Interdisciplinary Graduate Program in Immunology (EMC, SMV); University of Iowa, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- Toxicologic pathology, Vol.37(2), pp.249-255
- DOI
- 10.1177/0192623308329342
- PMID
- 19181630
- PMCID
- PMC2664312
- NLM abbreviation
- Toxicol Pathol
- ISSN
- 0192-6233
- eISSN
- 1533-1601
- Language
- English
- Date published
- 2009
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083886502771
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