Journal article
Comparison of histochemical methods for the analysis of eosinophils and mast cells using a porcine model of eosinophilic esophagitis
Frontiers in veterinary science, Vol.12, 1540995
03/19/2025
DOI: 10.3389/fvets.2025.1540995
PMCID: PMC11963769
PMID: 40177668
Abstract
Introduction: Accurate identification of eosinophils in tissue sections is required for diagnosis of eosinophilic esophagitis in humans and the assessment of severity of disease in allergy models. The pig may be a good model for sensitization and allergy models due to anatomical, physiological, and immunological similarities to humans. However, comparative studies on histochemical detection of eosinophils in fixed porcine tissue are lacking.
Methods: Qualitative and quantitative comparisons were performed for six histochemical methods previously reported for eosinophil and mast cell detection in other species. Astra Blue/Vital New Red, Congo Red, Luna, Sirius Red, Toluidine Blue, and modified regressive Hematoxylin & Eosin were applied to formalin-fixed paraffin embedded full-thickness sections of porcine esophagus. Specimens were collected from young, crossbred pigs sensitized to ovalbumin with or without subsequent oral exposure to ovalbumin to produce eosinophilic esophagitis lesions for comparison to non-allergic controls.
Results: Ease of eosinophil quantitation was analyzed, and varied by histochemical stain, to determine whether stain selection increased accuracy and efficiency of evaluation. Noticeable differences in color contrast between intracytoplasmic granules, surrounding tissue, and cellular components aided detection and identification of eosinophils and mast cells with Astra Blue/New Vital Red and Toluidine Blue, respectively. For eosinophils, Congo Red and H&E were adequate, while Luna and Sirius Red presented challenges for quantitation.
Discussion: In this case, rapid and reliable characterization of porcine esophageal allergy models was made possible by using Astra Blue/New Vital Red for eosinophils and Toluidine Blue for mast cells.
Details
- Title: Subtitle
- Comparison of histochemical methods for the analysis of eosinophils and mast cells using a porcine model of eosinophilic esophagitis
- Creators
- Douglas B. Snider - North Carolina State UniversityDavid K. Meyerholz - University of IowaEvan S. Dellon - University of North Carolina at Chapel HillLizette M. Cortes - North Carolina State UniversityAkash Karri - North Carolina State UniversityAnthony T. Blikslager - North Carolina State UniversityScott Laster - North Carolina State UniversityTobias Käser - North Carolina State UniversityGlenn Cruse - North Carolina State University
- Resource Type
- Journal article
- Publication Details
- Frontiers in veterinary science, Vol.12, 1540995
- DOI
- 10.3389/fvets.2025.1540995
- PMID
- 40177668
- PMCID
- PMC11963769
- NLM abbreviation
- Front Vet Sci
- ISSN
- 2297-1769
- eISSN
- 2297-1769
- Publisher
- FRONTIERS MEDIA SA
- Grant note
- Institutional National Research Service Award from the Office of the Director of National Institutes of Health NIH: 2T32OD011130 National Institutes of Health: HL091842, HL152960, DK054759 NIH: DK 034987, 1R21AI149098-01 NIAID: R01AI143985 Department of Molecular Biomedical Sciences (GC)Department of Population Health and Pathobiology (TK), College of Veterinary Medicine, North Carolina State University
The author(s) declare that financial support was received for the research and/or publication of this article. Research reported in this publication was supported Institutional National Research Service Award from the Office of the Director of National Institutes of Health NIH 2T32OD011130 (DS), National Institutes of Health grants HL091842 (DM), HL152960 (DM), DK054759 (DM), NIH P30 grant to the Center for Gastrointestinal Biology and Disease (CGIBD) (DK 034987) (TK), NIH R21 Exploratory/Developmental grant 1R21AI149098-01 (SL and TK), NIAID RO1 grant R01AI143985 (GC), start-up funds from the Department of Molecular Biomedical Sciences (GC) and the Department of Population Health and Pathobiology (TK), College of Veterinary Medicine, North Carolina State University, and histochemical staining support from Division of Comparative Pathology, Carver College of Medicine, University of Iowa (DM).
- Language
- English
- Date published
- 03/19/2025
- Academic Unit
- Pathology
- Record Identifier
- 9984802502202771
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