Comparison of multiple red cell volume methods performed concurrently in premature infants following allogeneic transfusion

Demet Nalbant; Prasad Bhandary; Nell I Matthews; Robert L Schmidt; Anna Bogusiewicz; Gretchen A Cress; M Bridget Zimmerman; Ronald G Strauss; Donald M Mock; John A Widness

doi:10.1038/pr.2013.143

Back

Comparison of multiple red cell volume methods performed concurrently in premature infants following allogeneic transfusion

Journal article

Open access

Peer reviewed

Comparison of multiple red cell volume methods performed concurrently in premature infants following allogeneic transfusion

Demet Nalbant, Prasad Bhandary, Nell I Matthews, Robert L Schmidt, Anna Bogusiewicz, Gretchen A Cress, M Bridget Zimmerman, Ronald G Strauss, Donald M Mock and John A Widness

Pediatric research, Vol.74(5), pp.592-600

11/2013

DOI: 10.1038/pr.2013.143

PMCID: PMC3907947

PMID: 24088873

Files and links (1)

url

https://doi.org/10.1038/pr.2013.143View

Published (Version of record) Open Access

Abstract

Study of the pathophysiology and treatment of anemia of prematurity is facilitated by direct measurement of red cell volume (RCV) utilizing microliter quantities of blood samples. Our objective was to compare concurrent measurements of multiple direct RCV methods in infants. Eighteen preterm infants receiving clinically indicated transfusions had concurrent flow cytometric determinations of RCV and 24-h red blood cell (RBC) recovery based on donor-recipient differences of biotin-labeled RBCs (BioRBCs), Kidd antigen mismatched RBCs, and fetal hemoglobin-positive (HbF(+)) RBCs. High-performance liquid chromatography (HPLC) was also used for measuring HbF and adult hemoglobin protein concentrations for the determination of RCV. Concurrent RCV measurements using BioRBCs (18 and 54 µg/ml), Kidd antigen, and HbF flow cytometry were not statistically different compared with RCVs measured using the reference BioRBC density (6 µg/ml). By contrast, the HbF-HPLC method overestimated RCV by 45% compared with the reference method. All the methods demonstrated 100% 24-h posttransfusion RBC recovery (PTR24). Because BioRBC, Kidd antigen, and fetal hemoglobin (HbF) flow cytometry are safe and accurate methods requiring <10 µl of patient blood for determining RCV and PTR24 in preterm infants, they can be useful in clinical and research studies of anemia and other conditions.

Erythrocyte Volume

Kidd Blood-Group System - analysis

Regression Analysis

Flow Cytometry - methods

Chromatography, High Pressure Liquid - methods

Infant, Premature - blood

Humans

Anemia - therapy

Infant, Newborn

Infant, Very Low Birth Weight - blood

Blood Transfusion - methods

Fetal Hemoglobin - metabolism

Details

Title: Subtitle: Comparison of multiple red cell volume methods performed concurrently in premature infants following allogeneic transfusion
Creators: Demet Nalbant - Department of Pediatrics, University of Iowa, Iowa City, Iowa
Prasad Bhandary
Nell I Matthews
Robert L Schmidt
Anna Bogusiewicz
Gretchen A Cress
M Bridget Zimmerman
Ronald G Strauss
Donald M Mock
John A Widness
Resource Type: Journal article
Publication Details: Pediatric research, Vol.74(5), pp.592-600
Publisher: United States
DOI: 10.1038/pr.2013.143
PMID: 24088873
PMCID: PMC3907947
ISSN: 0031-3998
eISSN: 1530-0447
Grant note: P01 HL046925 / NHLBI NIH HHS UL1 RR024979 / NCRR NIH HHS UL1RR024979 / NCRR NIH HHS
Language: English
Date published: 11/2013
Academic Unit: Stead Family Department of Pediatrics; Pathology; Pharmaceutical Sciences and Experimental Therapeutics; Biostatistics; Gastroenterology, Hepatology, Pancreatology, and Nutrition
Record Identifier: 9983997483902771

Metrics

32 Record Views

13 Times Cited - Web of Science