Comparison of the Effects of High-Fat Diet on Energy Flux in Mice Using Two Multiplexed Metabolic Phenotyping Systems

Jamie E Soto; Colin M L Burnett; Patrick Ten Eyck; E Dale Abel; Justin L Grobe

doi:10.1002/oby.22441

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Comparison of the Effects of High-Fat Diet on Energy Flux in Mice Using Two Multiplexed Metabolic Phenotyping Systems

Journal article

Peer reviewed

Comparison of the Effects of High-Fat Diet on Energy Flux in Mice Using Two Multiplexed Metabolic Phenotyping Systems

Jamie E Soto, Colin M L Burnett, Patrick Ten Eyck, E Dale Abel and Justin L Grobe

Obesity (Silver Spring, Md.), Vol.27(5), pp.793-802

05/2019

DOI: 10.1002/oby.22441

PMCID: PMC6478533

PMID: 30938081

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Abstract

Multiplexed metabolic phenotyping systems are available from multiple commercial vendors, and each system includes unique design features. Although expert opinion supports strengths and weaknesses of each design, empirical data from carefully controlled studies to test the biological impact of design differences are lacking. Wild-type C57BL/6J mice of both sexes underwent phenotyping in OxyMax (Columbus Instruments International) and Promethion (Sable Systems International) systems located within the same room of a newly constructed animal research facility in a crossover design study. Phenotypes were examined under chow (2920×)-fed conditions and again after 4 weeks of 60% high-fat diet (D12492) feeding. Food intake, physical activity, and respiratory gas exchange data significantly diverged between systems, depending upon sex of animals and diet supplied. Estimates of energy expenditure based on gas exchange in both systems accounted for a fraction of consumed calories that was greater in males than females. Design differences quantitatively impact the assessment of metabolic end points and thus the qualitative interpretation of various interventions. Importantly, current multiplexed systems remain blind to multiple additional end points, including digestive efficiency and selected forms of energy flux (nitrogenous, anaerobic, etc.), that account for a physiologically and/or pathophysiologically significant fraction of total energy flux.

Energy Intake - physiology

Phenotype

Animals

Mice, Inbred C57BL

Female

Male

Mice

Diet, High-Fat - methods

Details

Title: Subtitle: Comparison of the Effects of High-Fat Diet on Energy Flux in Mice Using Two Multiplexed Metabolic Phenotyping Systems
Creators: Jamie E Soto - Fraternal Order of Eagles' Diabetes Research Center, University of Iowa, Iowa City, Iowa, USA
Colin M L Burnett - Department of Pharmacology, University of Iowa, Iowa City, Iowa, USA
Patrick Ten Eyck - Institute for Clinical and Translational Science, University of Iowa, Iowa City, Iowa, USA
E Dale Abel - Abboud Cardiovascular Research Center, University of Iowa, Iowa City, Iowa, USA
Justin L Grobe - Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
Resource Type: Journal article
Publication Details: Obesity (Silver Spring, Md.), Vol.27(5), pp.793-802
DOI: 10.1002/oby.22441
PMID: 30938081
PMCID: PMC6478533
NLM abbreviation: Obesity (Silver Spring)
ISSN: 1930-7381
eISSN: 1930-739X
Publisher: United States
Grant note: R01 HL127764 / NHLBI NIH HHS UL1 TR002537 / NCATS NIH HHS P01 HL084207 / NHLBI NIH HHS R01 HL112413 / NHLBI NIH HHS R01 HL134850 / NHLBI NIH HHS K99 HL098276 / NHLBI NIH HHS
Language: English
Date published: 05/2019
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Biostatistics; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984024521902771

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