Comparison of the Vitek Gram-Positive Susceptibility 106 card and the MRSA-screen latex agglutination test for determining oxacillin resistance in clinical bloodstream isolates of Staphylococcus aureus

T Yamazumi; S A Marshall; W W Wilke; D J Diekema; M A Pfaller; R N Jones

doi:10.1128/JCM.39.1.53-56.2001

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Comparison of the Vitek Gram-Positive Susceptibility 106 card and the MRSA-screen latex agglutination test for determining oxacillin resistance in clinical bloodstream isolates of Staphylococcus aureus

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Comparison of the Vitek Gram-Positive Susceptibility 106 card and the MRSA-screen latex agglutination test for determining oxacillin resistance in clinical bloodstream isolates of Staphylococcus aureus

T Yamazumi, S A Marshall, W W Wilke, D J Diekema, M A Pfaller and R N Jones

Journal of clinical microbiology, Vol.39(1), pp.53-56

01/2001

DOI: 10.1128/JCM.39.1.53-56.2001

PMCID: PMC87679

PMID: 11136748

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Abstract

The Vitek automated susceptibility testing system with a modified Gram-Positive Susceptibility (GPS) 106 Card (bioMerieux Vitek, Inc., Hazelwood, Mo.) and a rapid slide latex agglutination test (MRSA-Screen; Denka Seiken Co., Ltd., Tokyo, Japan) were evaluated for their ability to detect oxacillin resistance in Staphylococcus aureus. The oxacillin-salt agar screen (OS) test, the reference broth microdilution method, and the detection of the mecA gene by PCR were compared with the commercial products. A total of 200 contemporary (1999) bloodstream infection isolates were collected from the SENTRY Antimicrobial Surveillance Program, representing diverse geographic areas throughout the world. Among the 99 mecA-positive isolates, 3 isolates were found negative by the MRSA-Screen. Another two isolates did not grow on OS plates and had MICs of 0.5 and 2 microg/ml with the Vitek GPS card. All 101 mecA-negative isolates were also found negative by the MRSA-Screen and were categorized as susceptible by the GPS card. Overall, the MRSA-Screen, GPS card, and OS test had sensitivities of 96.9, 98.0, and 98.0% and specificities of 100.0, 100.0, and 98.0%, respectively. MRSA-Screen was a rapid (</=15 min) and simple test to perform, and the GPS card provided results in <8 h. Both methods were sensitive and specific for detecting staphylococcal oxacillin resistance in the clinical microbiology laboratory.

Bacteremia - microbiology

Drug Resistance, Microbial

Methicillin Resistance - genetics

Staphylococcus aureus - isolation & purification

Latex Fixation Tests

Penicillin Resistance

Humans

Oxacillin - pharmacology

Staphylococcal Infections - microbiology

Penicillins - pharmacology

Staphylococcus aureus - drug effects

Microbial Sensitivity Tests - methods

Details

Title: Subtitle: Comparison of the Vitek Gram-Positive Susceptibility 106 card and the MRSA-screen latex agglutination test for determining oxacillin resistance in clinical bloodstream isolates of Staphylococcus aureus
Creators: T Yamazumi - Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
S A Marshall
W W Wilke
D J Diekema
M A Pfaller
R N Jones
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.39(1), pp.53-56
Publisher: United States
DOI: 10.1128/JCM.39.1.53-56.2001
PMID: 11136748
PMCID: PMC87679
ISSN: 0095-1137
eISSN: 1098-660X
Language: English
Date published: 01/2001
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986261702771

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