Comparison of the Vitek gram-positive susceptibility 106 card, the MRSA-Screen latex agglutination test, and mecA analysis for detecting oxacillin resistance in a geographically diverse collection of clinical isolates of coagulase-negative staphylococci

T Yamazumi; I Furuta; D J Diekema; M A Pfaller; R N Jones

doi:10.1128/JCM.39.10.3633-3636.2001

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Comparison of the Vitek gram-positive susceptibility 106 card, the MRSA-Screen latex agglutination test, and mecA analysis for detecting oxacillin resistance in a geographically diverse collection of clinical isolates of coagulase-negative staphylococci

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Comparison of the Vitek gram-positive susceptibility 106 card, the MRSA-Screen latex agglutination test, and mecA analysis for detecting oxacillin resistance in a geographically diverse collection of clinical isolates of coagulase-negative staphylococci

T Yamazumi, I Furuta, D J Diekema, M A Pfaller and R N Jones

Journal of clinical microbiology, Vol.39(10), pp.3633-3636

10/2001

DOI: 10.1128/JCM.39.10.3633-3636.2001

PMCID: PMC88400

PMID: 11574584

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Abstract

The Vitek automated susceptibility testing system with a modified gram-positive susceptibility (GPS) 106 card (bioMerieux Vitek, Inc., Hazelwood. Mo.) and a rapid slide latex agglutination test (MRSA-Screen test; Denka Seiken Co., Ltd., Tokyo, Japan) were evaluated for their abilities to detect oxacillin resistance in coagulase-negative staphylococci (CoNS). The reference broth microdilution method and the detection of the mecA gene by PCR ("gold standard" reference result) were used to compare the results obtained with the commercial products. A total of 123 clinical isolates consisting of eight species were selected from U.S. surveillance collections. Among the mecA-positive isolates (95 strains), 30 isolates were initially negative on the MRSA-Screen test read at 3 min. When the agglutination reaction was extended for 10 min, 26 of the 30 isolates became positive. For a different four isolates, the oxacillin MIC was < or =0.25 microg/ml on the Vitek GPS 106 card. Among the mecA-negative isolates (28 strains), for two Staphylococcus warneri, two S. lugdunensis, and two S. saprophyticus strains MICs were > or =0.5 microg/ml by the reference broth microdilution method. Four of these isolates were also categorized as resistant with the Vitek GPS 106 card and two isolates were positive by the MRSA-Screen test. Overall, the MRSA-Screen test, GPS 106 card, and reference broth microdilution method had sensitivities of 95.7 (result at 10 min), 95.7, and 100%, respectively, and specificities of 92.8, 85.7, and 78.5%, respectively. Although the MRSA-Screen test required a slight procedural modification, both commercial methods achieved a sensitivity and specificity at detecting oxacillin resistance in CoNS at a level that was acceptable for clinical laboratory use.

Peptidyl Transferases

Latex Fixation Tests

Humans

Oxacillin - pharmacology

Staphylococcus - drug effects

Bacterial Proteins

Coagulase - metabolism

Carrier Proteins - genetics

Penicillin-Binding Proteins

Staphylococcus - enzymology

Methicillin Resistance - genetics

Penicillin Resistance

Polymerase Chain Reaction

Hexosyltransferases

Staphylococcal Infections - microbiology

Muramoylpentapeptide Carboxypeptidase - genetics

Penicillins - pharmacology

Staphylococcus - genetics

Microbial Sensitivity Tests - methods

Details

Title: Subtitle: Comparison of the Vitek gram-positive susceptibility 106 card, the MRSA-Screen latex agglutination test, and mecA analysis for detecting oxacillin resistance in a geographically diverse collection of clinical isolates of coagulase-negative staphylococci
Creators: T Yamazumi - Medical Microbiology Division, Department of Pathology University of Iowa College of Medicine, Iowa City, Iowa, USA
I Furuta
D J Diekema
M A Pfaller
R N Jones
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.39(10), pp.3633-3636
DOI: 10.1128/JCM.39.10.3633-3636.2001
PMID: 11574584
PMCID: PMC88400
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 10/2001
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986366402771

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