Journal article
Comparison of the therapeutic effects of sildenafil citrate, heparin and neuropeptides in a rat model of acetic acid-induced gastric ulcer
Life sciences (1973), Vol.186, pp.102-110
10/01/2017
DOI: 10.1016/j.lfs.2017.08.013
PMID: 28807720
Abstract
The purpose of our investigative work has been to determine whether there can be therapeutic roles in the administration of sildenafil citrate, heparin and several neuropeptides on an animal model where gastric ulcers were induced with acetic acid, and to compare their efficacy.
The animals were divided into 13 groups, with 4 animals in each. Gastric ulcers was induced in the animals of 12 groups with one untreated group being left as the control (Group I - control; given normal saline (NS)). The other groups were: Group II (ulcer+NS); Group III (5mg/kg sildenafil citrate, low dose); Group IV (10mg/kg sildenafil citrate, high dose); Group V (0.6mg/kg heparin, low dose); Group VI (6mg/kg heparin, high dose); Group VII (20nmol/kg des-acyl ghrelin); Group VIII (40nmol/kg des-acyl ghrelin); Group IX (4nmol/kg acyl ghrelin); Group X (8nmol/kg acly ghrelin); Group XI (20pmol/kg Nesfatin-1); Group XII (15nmol/kg Obestatin) and Group XIII (5nmol/kg Neuropeptide Y). Gastric neuropeptide expression was measured using an immunohistochemical method, and the amount in circulation was detected using ELISA. To compare with no treatment, the controls and other treatment groups, we recorded loss of the surface epithelium of the stomach, erosion, bleeding and inflammatory cell infiltration in the upper halves of the gastric glands.
The muscularis and the layers beneath it were, however, apparently normal. The gastric mucosa healed with little or no inflammation when sildenafil citrate, low dose heparin, ghrelin, NUCB2/Nesfatin-1, obestatin, Neuropeptide Y were administered.
Overall the data indicate that low dose heparin, and especially sildenafil citrate and neuropeptides, can be used clinically as an alternative approach in the treatment of the gastric ulcer.
•Sildenafil citrate is the most promising agent in the treatment of gastric (peptic) ulcer.•Neuropeptides can be important among the options for the treatment of gastric ulcers.•Although heparin has the potential to treat the gastric ulcer, it is the weakest option.•Nesfatin-1, Obestatin and Neuropeptide Y decrease in the circulation and the gastric tissue when ulcers are present.
Details
- Title: Subtitle
- Comparison of the therapeutic effects of sildenafil citrate, heparin and neuropeptides in a rat model of acetic acid-induced gastric ulcer
- Creators
- Mehmet Kalayci - Department of Medical Biochemistry and Clinical Biochemistry, (Firat Hormones Research Group), Medical School, Firat University, 23119 Elazig, TurkeyMehmet Ali Kocdor - Dokuz Eylül UniversityTuncay Kuloglu - Fırat Universityİbrahim Sahin - Fırat UniversityMehmet Sarac - Fırat UniversityAziz Aksoy - Bitlis Eren UniversityMeltem Yardim - Department of Medical Biochemistry and Clinical Biochemistry, (Firat Hormones Research Group), Medical School, Firat University, 23119 Elazig, TurkeySemih Dalkilic - Department of Molecular Biology, Life Science Faculty, Firat University, 23119 Elazig, TurkeyOnur Gursu - Ümraniye Eğitim ve Araştırma HastanesiSuna Aydin - Department of Anatomy, Medical School, Firat University, 23119 Elazig, TurkeyRamazan Fazil Akkoc - Yeditepe UniversityMeltem Ugras - Yeditepe UniversityGokhan Artas - Fırat Universityİbrahim Hanifi Ozercan - Fırat UniversityKader Ugur - Fırat UniversitySuleyman Aydin - Fırat University
- Resource Type
- Journal article
- Publication Details
- Life sciences (1973), Vol.186, pp.102-110
- DOI
- 10.1016/j.lfs.2017.08.013
- PMID
- 28807720
- ISSN
- 0024-3205
- eISSN
- 1879-0631
- Publisher
- Elsevier Inc
- Number of pages
- 9
- Language
- English
- Date published
- 10/01/2017
- Academic Unit
- Stead Family Department of Pediatrics; Gastroenterology, Hepatology, Pancreatology, and Nutrition
- Record Identifier
- 9985214103302771
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