Journal article
Compartmentalization of Bacterial Antigens: Differential Effects on Priming of CD8 T Cells and Protective Immunity
Cell (Cambridge), Vol.92(4), pp.535-545
1998
DOI: 10.1016/S0092-8674(00)80946-0
PMID: 9491894
Abstract
Bacterial pathogens synthesize numerous proteins that are either secreted or localized within bacterial cells. To address the impact of antigen compartmentalization on T cell immunity, we constructed recombinant
Listeria monocytogenes that express a model CD8 T cell epitope as a secreted or nonsecreted fusion protein. Both forms of the antigen, either secreted into the host cell cytoplasm or retained within bacterial cells, efficiently prime CD8 T cell responses. However, epitope-specific CD8 T cells confer protection only against bacteria secreting the antigen but not against the bacteria expressing the nonsecreted form of the same antigen. This dichotomy as a result of antigen compartmentalization suggests that bacterial antigens are presented by multiple MHC class I pathways to prime CD8 T cells, but only the endogenous pathway provides target antigens for CD8 T cell–mediated protective immunity.
Details
- Title: Subtitle
- Compartmentalization of Bacterial Antigens: Differential Effects on Priming of CD8 T Cells and Protective Immunity
- Creators
- Hao Shen - Emory Vaccine Center, Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USAJeff F Miller - Department of Microbiology and Immunology, UCLA School of Medicine, Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, USAXin Fan - Department of Microbiology and Immunology, UCLA School of Medicine, Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, USADavid Kolwyck - Department of Microbiology, Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USARafi Ahmed - Emory Vaccine Center, Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USAJohn T Harty - Department of Microbiology, Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USA
- Resource Type
- Journal article
- Publication Details
- Cell (Cambridge), Vol.92(4), pp.535-545
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0092-8674(00)80946-0
- PMID
- 9491894
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Language
- English
- Date published
- 1998
- Academic Unit
- Pathology
- Record Identifier
- 9984047721802771
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