Journal article
Compartmentalization of Hepatitis C Virus (HCV) during HCV/HIV Coinfection
The Journal of infectious diseases, Vol.195(12), pp.1765-1773
06/15/2007
DOI: 10.1086/518251
PMCID: PMC3210030
PMID: 17492592
Abstract
Extrahepatic replication has important implications for the transmission and treatment of hepatitis C virus (HCV). We analyzed longitudinal HCV diversity in peripheral-blood mononuclear cells (PBMCs) and serum during HCV monoinfection and HCV/HIV coinfection to determine whether distinct amino acid signatures characterized HCV replicating within PBMCs. Analysis of E1-HVR1 sequences demonstrated higher serum genetic distances among HCV/human immunodeficiency virus (HIV)—coinfected persons. Moreover, consensus PBMC sequences were rarely identical to those in the corresponding serum, suggesting divergence in these 2 compartments. Three of 5 HCV/HIV-coinfected participants showed evidence of HCV compartmentalization in PBMCs. Additionally, signature sequence analysis identified PBMC-specific amino acids in all HCV/HIVcoinfected persons. To our knowledge, this is the first study to identify specific amino acids that may distinguish HCV variants replicating in PBMCs. It is provocative to speculate that extrahepatic HCV diversity may be an important determinant of treatment response and thus warrants additional study, particularly during HCV/ HIV coinfection.
Details
- Title: Subtitle
- Compartmentalization of Hepatitis C Virus (HCV) during HCV/HIV Coinfection
- Creators
- Jason T. Blackard - University of Cincinnati Medical CenterYoichi Hiasa - Ehime UniversityLaura Smeaton - Cancer Research And BiostatisticsDenise J. Jamieson - Centers for Disease Control and PreventionIrma Rodriguez - Miriam HospitalKenneth H. Mayer - Brown UniversityRaymond T. Chung - Massachusetts General Hospital
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.195(12), pp.1765-1773
- Publisher
- The University of Chicago Press
- DOI
- 10.1086/518251
- PMID
- 17492592
- PMCID
- PMC3210030
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 06/15/2007
- Academic Unit
- Obstetrics and Gynecology; VPMA - Administration
- Record Identifier
- 9984446538702771
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