Journal article
Compensatory activation of the multidrug transporters Pdr5p, Snq2p, and Yor1p by Pdr1p in Saccharomyces cerevisiae
FEBS letters, Vol.582(6), pp.977-983
03/19/2008
DOI: 10.1016/j.febslet.2008.02.045
PMCID: PMC2288637
PMID: 18307995
Abstract
In Saccharomyces cerevisiae, the transcription factors Pdr1p and Pdr3p activate the expression of several genes, including PDR5, SNQ2, and YOR1, which encode ATP-binding cassette transporters that extrude dozens of antifungals with overlapping but distinct specificity. In this study, it was observed that growth resistance to specific Pdr5p substrates rose upon disruption of the YOR1 or SNQ2 coding region and was accompanied by increased efflux. Similarly, resistance to Yor1p- and Snq2p-specific substrates increased upon deletion of PDR5. The mRNA and protein levels of the respective transporters increased in parallel to drug resistance. beta-Galactosidase activity fused to the PDR5 or YOR1 promoter required the presence of Pdr1p and its specific binding sites for the compensatory induction, whereas Pdr3p had an inhibitory effect. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Details
- Title: Subtitle
- Compensatory activation of the multidrug transporters Pdr5p, Snq2p, and Yor1p by Pdr1p in Saccharomyces cerevisiae
- Creators
- Anna Kolaczkowska - University of WrocławMarcin Kolaczkowski - Wrocław Medical UniversityAndre Goffeau - Université Catholique de LouvainW. Scott Moye-Rowley - University of Iowa
- Resource Type
- Journal article
- Publication Details
- FEBS letters, Vol.582(6), pp.977-983
- DOI
- 10.1016/j.febslet.2008.02.045
- PMID
- 18307995
- PMCID
- PMC2288637
- NLM abbreviation
- FEBS Lett
- ISSN
- 1873-3468
- eISSN
- 1873-3468
- Publisher
- Wiley
- Number of pages
- 7
- Grant note
- R01GM049825 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01 GM049825; R01 GM049825-11; GM49825 / NIGMS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) Intramural NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 03/19/2008
- Academic Unit
- Molecular Physiology and Biophysics; Internal Medicine
- Record Identifier
- 9984297492302771
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