Journal article
Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-& gamma; signal transduction pathway
Nature (London), Vol.366(6451), pp.166-170
11/1993
DOI: 10.1038/366166a0
PMID: 7901766
Abstract
INTERFERONS (IFNs) & alpha;/& beta; (type I) and & gamma; (type II) bind to distinct cell surface receptors1, inducing transcription of overlapping sets of genes by intracellular pathways that have recently attracted much attention2,3. Previous studies using cell lines selected for their inability to respond to IFN-& alpha; (ref. 4) have shown that the protein kinase Tyk2 plays a central role in the IFN & alpha;/& beta; response5. Here we report the isolation of the cell line & gamma;l A, selected for its inability to express IFN-& gamma;-inducible cell-surface markers, that is deficient in all aspects of the IFN-& gamma; response tested, but responds normally to IFNs & alpha; and & beta;. The mutant cells can be complemented by the expression of another member of the JAK family of protein tyro-sine kinases, JAK2 (refs 6& ndash;9). Unlike IFNs & alpha; and & beta;, IFN-& gamma; induces rapid tyrosine phosphorylation of JAK2 in wild-type cells, and JAK2 immunoprecipitates from these cells show tyrosine kinase activity. These responses are absent in & gamma;l A cells. JAK2 is therefore required for the response to IFN-& gamma; but not to IFNs & alpha; and & beta;.
Details
- Title: Subtitle
- Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-& gamma; signal transduction pathway
- Creators
- Diane WatlingDmitry GuschinMathias MüllerOlli SilvennoinenBruce A WitthuhnFrederick W QuelleNeil C RogersChris SchindlerGeorge R StarkJames N Ihlelan M Kerr
- Resource Type
- Journal article
- Publication Details
- Nature (London), Vol.366(6451), pp.166-170
- DOI
- 10.1038/366166a0
- PMID
- 7901766
- ISSN
- 0028-0836
- eISSN
- 1476-4687
- Language
- English
- Date published
- 11/1993
- Academic Unit
- Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040540802771
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