Journal article
Complete Anopheles funestus mitogenomes reveal an ancient history of mitochondrial lineages and their distribution in southern and central Africa
Scientific reports, Vol.8(1), pp.9054-11
06/13/2018
DOI: 10.1038/s41598-018-27092-y
PMCID: PMC5997999
PMID: 29899497
Abstract
Anopheles funestus s.s. is a primary vector of malaria in sub-Saharan Africa. Despite its important role in human Plasmodium transmission, evolutionary history, genetic diversity, and population structure of An. funestus in southern and central Africa remains understudied. We deep sequenced, assembled, and annotated the complete mitochondrial genome of An. funestus s.s. for the first time, providing a foundation for further genetic research of this important malaria vector species. We further analyzed the complete mitochondrial genomes of 43 An. funestus s.s. from three sites in Zambia, Democratic Republic of the Congo, and Tanzania. From these 43 mitogenomes we identified 41 unique haplotypes that comprised 567 polymorphic sites. Bayesian phylogenetic reconstruction confirmed the coexistence of two highly divergent An. funestus maternal lineages, herein defined as lineages I and II, in Zambia and Tanzania. The estimated coalescence time of these two mitochondrial lineages is similar to 500,000 years ago (95% HPD 426,000-594,000 years ago) with subsequent independent diversification. Haplotype network and phylogenetic analysis revealed two major clusters within lineage I, and genetic relatedness of samples with deep branching in lineage II. At this time, data suggest that the lineages are partially sympatric. This study illustrates that accurate retrieval of full mitogenomes of Anopheles vectors enables fine-resolution studies of intraspecies genetic relationships, population differentiation, and demographic history. Further investigations on whether An. funestus mitochondrial lineages represent biologically meaningful populations and their potential implications for malaria vector control are warranted.
Details
- Title: Subtitle
- Complete Anopheles funestus mitogenomes reveal an ancient history of mitochondrial lineages and their distribution in southern and central Africa
- Creators
- Christine M. Jones - Johns Hopkins UniversityYoosook Lee - University of California, DavisAndrew Kitchen - University of IowaTravis Collier - Agricultural Research ServiceJulia C. Pringle - Johns Hopkins UniversityMbanga Muleba - MBIASeth Irish - Centers for Disease Control and PreventionJennifer C. Stevenson - Johns Hopkins UniversityMaureen Coetzee - University of the WitwatersrandAnthony J. Cornel - University of California, DavisDouglas E. Norris - Johns Hopkins UniversityGiovanna Carpi - Johns Hopkins University
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.8(1), pp.9054-11
- DOI
- 10.1038/s41598-018-27092-y
- PMID
- 29899497
- PMCID
- PMC5997999
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Publisher
- Springer Nature
- Number of pages
- 11
- Grant note
- 2U19AI089680 / National Institutes of Health as part of the International Centers of Excellence for Malaria Research; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Johns Hopkins Malaria Research Institute T32AI007417 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) DST/NRF Research Chair Initiative Bloomberg Philanthropies President's Malaria Initiative; United States Agency for International Development (USAID); President's Malaria Initiative (PMI) T32AI007417 / T32 grant
- Language
- English
- Date published
- 06/13/2018
- Academic Unit
- Anthropology; International Programs
- Record Identifier
- 9984271555802771
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