Compositional differences between infant and adult human corneal basement membranes

Andrea Kabosova; Dimitri T Azar; Gregory A Bannikov; Kevin P Campbell; Madeleine Durbeej; Reza F Ghohestani; Jonathan C R Jones; M Cristina Kenney; Manuel Koch; Yoshifumi Ninomiya; Bruce L Patton; Mats Paulsson; Yoshikazu Sado; E Helene Sage; Takako Sasaki; Lydia M Sorokin; Marie-France Steiner-Champliaud; Tung-Tien Sun; Nirmala Sundarraj; Rupert Timpl; Ismo Virtanen; Alexander V Ljubimov

doi:10.1167/iovs.07-0654

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Compositional differences between infant and adult human corneal basement membranes

Journal article

Peer reviewed

Compositional differences between infant and adult human corneal basement membranes

Andrea Kabosova, Dimitri T Azar, Gregory A Bannikov, Kevin P Campbell, Madeleine Durbeej, Reza F Ghohestani, Jonathan C R Jones, M Cristina Kenney, Manuel Koch, Yoshifumi Ninomiya, …

Investigative ophthalmology & visual science, Vol.48(11), pp.4989-4999

11/2007

DOI: 10.1167/iovs.07-0654

PMCID: PMC2151758

PMID: 17962449

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Abstract

Adult human corneal epithelial basement membrane (EBM) and Descemet's membrane (DM) components exhibit heterogeneous distribution. The purpose of the study was to identify changes of these components during postnatal corneal development. Thirty healthy adult corneas and 10 corneas from 12-day- to 3-year-old children were studied by immunofluorescence with antibodies against BM components. Type IV collagen composition of infant corneal central EBM over Bowman's layer changed from alpha1-alpha2 to alpha3-alpha4 chains after 3 years of life; in the adult, alpha1-alpha2 chains were retained only in the limbal BM. Laminin alpha2 and beta2 chains were present in the adult limbal BM where epithelial stem cells are located. By 3 years of age, beta2 chain appeared in the limbal BM. In all corneas, limbal BM contained laminin gamma3 chain. In the infant DM, type IV collagen alpha1-alpha6 chains, perlecan, nidogen-1, nidogen-2, and netrin-4 were found on both faces, but they remained only on the endothelial face of the adult DM. The stromal face of the infant but not the adult DM was positive for tenascin-C, fibrillin-1, SPARC, and laminin-332. Type VIII collagen shifted from the endothelial face of infant DM to its stromal face in the adult. Matrilin-4 largely disappeared after the age of 3 years. The distribution of laminin gamma3 chain, nidogen-2, netrin-4, matrilin-2, and matrilin-4 is described in the cornea for the first time. The observed differences between adult and infant corneal BMs may relate to changes in their mechanical strength, corneal cell adhesion and differentiation in the process of postnatal corneal maturation.

Humans

Middle Aged

Child, Preschool

Infant

Antibodies, Monoclonal

Bowman Membrane - chemistry

Extracellular Matrix Proteins - analysis

Basement Membrane - chemistry

Adolescent

Adult

Aged

Descemet Membrane - chemistry

Infant, Newborn

Microscopy, Fluorescence

Details

Title: Subtitle: Compositional differences between infant and adult human corneal basement membranes
Creators: Andrea Kabosova - Ophthalmology Research Laboratories, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA
Dimitri T Azar
Gregory A Bannikov
Kevin P Campbell
Madeleine Durbeej
Reza F Ghohestani
Jonathan C R Jones
M Cristina Kenney
Manuel Koch
Yoshifumi Ninomiya
Bruce L Patton
Mats Paulsson
Yoshikazu Sado
E Helene Sage
Takako Sasaki
Lydia M Sorokin
Marie-France Steiner-Champliaud
Tung-Tien Sun
Nirmala Sundarraj
Rupert Timpl
Ismo Virtanen
Alexander V Ljubimov
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.48(11), pp.4989-4999
DOI: 10.1167/iovs.07-0654
PMID: 17962449
PMCID: PMC2151758
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Grant note: R01 EY013431-06 / NEI NIH HHS R01 EY10836 / NEI NIH HHS R01 AR054184 / NIAMS NIH HHS R01 EY13431 / NEI NIH HHS R01 EY013431 / NEI NIH HHS R01 AR054184-18 / NIAMS NIH HHS R01 AR054184-20 / NIAMS NIH HHS
Language: English
Date published: 11/2007
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984020850702771

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