Comprehensive analysis of microRNA signature of mouse pancreatic acini: overexpression of miR-21-3p in acute pancreatitis

Ajay Kumar Dixit; Anne E Sarver; Zuobiao Yuan; John George; Usman Barlass; Hassam Cheema; Archana Sareen; Sulagna Banerjee; Vikas Dudeja; Rajinder Dawra; Subbaya Subramanian; Ashok K Saluja

doi:10.1152/ajpgi.00191.2016

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Comprehensive analysis of microRNA signature of mouse pancreatic acini: overexpression of miR-21-3p in acute pancreatitis

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Comprehensive analysis of microRNA signature of mouse pancreatic acini: overexpression of miR-21-3p in acute pancreatitis

Ajay Kumar Dixit, Anne E Sarver, Zuobiao Yuan, John George, Usman Barlass, Hassam Cheema, Archana Sareen, Sulagna Banerjee, Vikas Dudeja, Rajinder Dawra, …

American journal of physiology: Gastrointestinal and liver physiology, Vol.311(5), pp.G974-G980

11/01/2016

DOI: 10.1152/ajpgi.00191.2016

PMID: 27686613

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Abstract

In the current study, we have characterized the global miRNA expression profile in mouse pancreatic acinar cells and during acute pancreatitis using next-generation RNA sequencing. We identified 324 known and six novel miRNAs that are expressed in mouse pancreatic acinar cells. In the basal state, miR-148a-3p, miR-375-3p, miR-217-5p, and miR-200a-3p were among the most abundantly expressed, whereas miR-24-5p and miR-421-3p were the least abundant. Treatment of acinar cells with caerulein (100 nM) and taurolithocholic acid 3-sulfate [TLC-S (250 μM)] induced numerous changes in miRNA expression profile. In particular, we found significant overexpression of miR-21-3p in acini treated with caerulein and TLC-S. We further looked at the expression of miR-21-3p in caerulein, l-arginine, and caerulein + LPS-induced acute pancreatitis mouse models and found 12-, 21-, and 50-fold increased expression in the pancreas, respectively. In summary, this is the first comprehensive analysis of global miRNA expression profile of mouse pancreatic acinar cells in normal and disease conditions. Our analysis shows that miR-21-3p expression level correlates with the severity of the disease.

Acinar Cells - drug effects

Acinar Cells - metabolism

Animals

Ceruletide - pharmacology

Gene Expression - drug effects

Gene Expression Profiling

High-Throughput Nucleotide Sequencing

Mice

MicroRNAs - genetics

MicroRNAs - metabolism

Pancreatitis - genetics

Pancreatitis - metabolism

Taurolithocholic Acid - analogs & derivatives

Taurolithocholic Acid - pharmacology

Details

Title: Subtitle: Comprehensive analysis of microRNA signature of mouse pancreatic acini: overexpression of miR-21-3p in acute pancreatitis
Creators: Ajay Kumar Dixit - University of Miami
Anne E Sarver - University of Minnesota
Zuobiao Yuan - University of Minnesota
John George - University of Miami
Usman Barlass - University of Minnesota
Hassam Cheema - University of Miami
Archana Sareen - University of Minnesota
Sulagna Banerjee - University of Miami
Vikas Dudeja - University of Miami
Rajinder Dawra - University of Miami
Subbaya Subramanian - University of Minnesota
Ashok K Saluja - University of Miami
Resource Type: Journal article
Publication Details: American journal of physiology: Gastrointestinal and liver physiology, Vol.311(5), pp.G974-G980
DOI: 10.1152/ajpgi.00191.2016
PMID: 27686613
NLM abbreviation: Am J Physiol Gastrointest Liver Physiol
ISSN: 0193-1857
eISSN: 1522-1547
Grant note: R01 DK092145 / NIDDK NIH HHS R01 DK058694 / NIDDK NIH HHS R01 DK093047 / NIDDK NIH HHS
Language: English
Date published: 11/01/2016
Academic Unit: Anatomy and Cell Biology; Surgery
Record Identifier: 9985244576002771

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