Journal article
Concentration of the antibacterial precursor thiocyanate in cystic fibrosis airway secretions
Free radical biology & medicine, Vol.50(9), pp.1144-1150
05/01/2011
DOI: 10.1016/j.freeradbiomed.2011.02.013
PMCID: PMC3070840
PMID: 21334431
Abstract
A recently discovered enzyme system produces antibacterial hypothiocyanite (OSCN−) in the airway lumen by oxidizing the secreted precursor thiocyanate (SCN−). Airway epithelial cultures have been shown to secrete SCN− in a CFTR-dependent manner. Thus, reduced SCN− availability in the airway might contribute to the pathogenesis of cystic fibrosis (CF), a disease caused by mutations in the CFTR gene and characterized by an airway host defense defect. We tested this hypothesis by analyzing the SCN− concentration in the nasal airway surface liquid (ASL) of CF patients and non-CF subjects and in the tracheobronchial ASL of CFTR-ΔF508 homozygous pigs and control littermates. In the nasal ASL, the SCN− concentration was ~30-fold higher than in serum independent of the CFTR mutation status of the human subject. In the tracheobronchial ASL of CF pigs, the SCN− concentration was somewhat reduced. Among human subjects, SCN− concentrations in the ASL varied from person to person independent of CFTR expression, and CF patients with high SCN− levels had better lung function than those with low SCN− levels. Thus, although CFTR can contribute to SCN− transport, it is not indispensable for the high SCN− concentration in ASL. The correlation between lung function and SCN− concentration in CF patients may reflect a beneficial role for SCN−.
Details
- Title: Subtitle
- Concentration of the antibacterial precursor thiocyanate in cystic fibrosis airway secretions
- Creators
- Daniel Lorentzen - Inflammation Program, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USALakshmi Durairaj - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAAlejandro A Pezzulo - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAYoko Nakano - Inflammation Program, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAJanice Launspach - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USADavid A Stoltz - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAGideon Zamba - Department of Biostatistics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAPaul B McCray - Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAJoseph Zabner - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAMichael J Welsh - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAWilliam M Nauseef - Inflammation Program, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USABotond Bánfi - Inflammation Program, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.50(9), pp.1144-1150
- DOI
- 10.1016/j.freeradbiomed.2011.02.013
- PMID
- 21334431
- PMCID
- PMC3070840
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100006501, name: Cystic Fibrosis Foundation Therapeutics, award: R458-CR02, BANFI07A0; DOI: 10.13039/100000050, name: NHLBI, award: HL090830, HL91842; name: NIH, award: HL075402; DOI: 10.13039/100000050, name: NHLBI, award: HL51670; DOI: 10.13039/100000897, name: Cystic Fibrosis Foundation, award: R458-CR02, ENGLH9850; DOI: 10.13039/100000062, name: NIDDK, award: DK54759
- Language
- English
- Date published
- 05/01/2011
- Academic Unit
- Neurology; Microbiology and Immunology; Biostatistics; Otolaryngology; Roy J. Carver Department of Biomedical Engineering; Radiology; Molecular Physiology and Biophysics; Pulmonary Medicine; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurosurgery; Internal Medicine
- Record Identifier
- 9983997444602771
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