Conditional Deletion of N-Myc Disrupts Neurosensory and Non-sensory Development of the Ear

Benjamin Kopecky; Peter Santi; Shane Johnson; Heather Schmitz; Bernd Fritzsch

doi:10.1002/dvdy.22620

Back

Conditional Deletion of N-Myc Disrupts Neurosensory and Non-sensory Development of the Ear

Journal article

Open access

Peer reviewed

Conditional Deletion of N-Myc Disrupts Neurosensory and Non-sensory Development of the Ear

Benjamin Kopecky, Peter Santi, Shane Johnson, Heather Schmitz and Bernd Fritzsch

Developmental dynamics, Vol.240(6), pp.1373-1390

06/2011

DOI: 10.1002/dvdy.22620

PMCID: PMC3092837

PMID: 21448975

Files and links (1)

url

https://doi.org/10.1002/dvdy.22620View

Published (Version of record) Open Access

Abstract

Ear development requires interactions of transcription factors for proliferation and differentiation. The proto-oncogene\nN-Myc\nis a member of the\nMyc\nfamily that regulate proliferation. To investigate the function of\nN-Myc\n, we conditionally knocked out\nN-Myc\nin the ear using\nTg(Pax2-Cre)\nand\nFoxg 1\nKiCre\n.\nN-Myc\nCKOs had reduced growth of the ear, abnormal morphology including fused sensory epithelia, disrupted histology, and disorganized neuronal innervation. Using Thin-Sheet Laser Imaging Microscopy (TSLIM), 3D reconstruction and quantification of the cochlea revealed a greater than fifty percent size reduction. Immunochemistry and\nin situ\nhybridization showed a gravistatic organ-cochlear fusion and a “circularized” apex with no clear inner and outer hair cells. Furthermore, the abnormally developed cochlea had cross innervation from the vestibular ganglion near the basal tip. These findings are put in the context of the possible functional relationship of\nN-Myc\nwith a number of other cell proliferative and fate determining genes during ear development.

Morphogenesis

Cell Cycle

N-Myc

Histogenesis

Inner Ear

Details

Title: Subtitle: Conditional Deletion of N-Myc Disrupts Neurosensory and Non-sensory Development of the Ear
Creators: Benjamin Kopecky - Department of Biology, University of Iowa, Iowa City, Iowa, USA
Peter Santi - Department of Otolaryngology, University of Minnesota, Minneapolis, MN, USA
Shane Johnson - Department of Otolaryngology, University of Minnesota, Minneapolis, MN, USA
Heather Schmitz - Department of Otolaryngology, University of Minnesota, Minneapolis, MN, USA
Bernd Fritzsch - Department of Biology, University of Iowa, Iowa City, Iowa, USA
Resource Type: Journal article
Publication Details: Developmental dynamics, Vol.240(6), pp.1373-1390
DOI: 10.1002/dvdy.22620
PMID: 21448975
PMCID: PMC3092837
NLM abbreviation: Dev Dyn
ISSN: 1058-8388
eISSN: 1097-0177
Language: English
Date published: 06/2011
Academic Unit: Psychological and Brain Sciences; Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070636102771

Metrics

23 Record Views

57 Times Cited - Web of Science