Conditional and inducible gene recombineering in the mouse inner ear

Yong Tian; Sally James; Jian Zuo; Bernd Fritzsch; Kirk W Beisel

doi:10.1016/j.brainres.2006.01.040

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Conditional and inducible gene recombineering in the mouse inner ear

Journal article

Peer reviewed

Conditional and inducible gene recombineering in the mouse inner ear

Yong Tian, Sally James, Jian Zuo, Bernd Fritzsch and Kirk W Beisel

Brain research, Vol.1091(1), pp.243-254

2006

DOI: 10.1016/j.brainres.2006.01.040

PMCID: PMC3901521

PMID: 16488403

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https://www.ncbi.nlm.nih.gov/pmc/articles/3901521View

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Abstract

Genetically engineered mice have greatly improved our understanding of gene functions and disease mechanisms. Nevertheless, the traditional knock-out approach has limitations in the overall viability of mutants. The application of the\nCre/loxP system in the inner ear can help bypass this difficulty by generation of conditional gene recombineering. However, to do so requires an expression system that allows ear-specific temporally inducible, gene abrogation of one or more of the increasingly available floxed genes. To date, three approaches have been successfully used to create murine inner ear-specific\nCre lines: conventional transgenesis, BAC transgenesis, and gene knock-in. Unfortunately, timing of conditional Cre activity does not extend beyond the regulatory range of the gene controlling Cre expression. Rectification of this problem requires the generation of tamoxifen or tetracycline inducible systems in the inner ear. Examination of integrase expression at different loci will facilitate studies on the expression of exogenous transgenes. These genetic applications for the mouse genome will dramatically advance in vivo gene function studies.

Integrase

Inducible

Recombinase

Transgene

Recombineering

Inner ear

Details

Title: Subtitle: Conditional and inducible gene recombineering in the mouse inner ear
Creators: Yong Tian - Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA
Sally James - Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA
Jian Zuo - Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA
Bernd Fritzsch - Department of Biomedical Sciences, Creighton University, School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA
Kirk W Beisel - Department of Biomedical Sciences, Creighton University, School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA
Resource Type: Journal article
Publication Details: Brain research, Vol.1091(1), pp.243-254
DOI: 10.1016/j.brainres.2006.01.040
PMID: 16488403
PMCID: PMC3901521
NLM abbreviation: Brain Res
ISSN: 0006-8993
eISSN: 1872-6240
Publisher: Elsevier B.V
Language: English
Date published: 2006
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070860402771

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