Conditional deletion of Sox17 reveals complex effects on uterine adenogenesis and function

Amy Guimarães-Young; Traci Neff; Adam J Dupuy; Michael J Goodheart

doi:10.1016/j.ydbio.2016.04.010

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Conditional deletion of Sox17 reveals complex effects on uterine adenogenesis and function

Journal article

Open access

Peer reviewed

Conditional deletion of Sox17 reveals complex effects on uterine adenogenesis and function

Amy Guimarães-Young, Traci Neff, Adam J Dupuy and Michael J Goodheart

Developmental Biology, Vol.414(2), pp.219-227

06/15/2016

DOI: 10.1016/j.ydbio.2016.04.010

PMCID: PMC5521196

PMID: 27102016

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https://doi.org/10.1016/j.ydbio.2016.04.010View

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Abstract

The importance of canonical Wnt signaling to murine uterine development is well established. Mouse models in which uterine-specific Wnt ligands, β-catenin, or Lef1 are disrupted result in failure of postnatal endometrial gland development. Sox17 is a transcription factor characterized in numerous tissues as an antagonist of Wnt signaling. Thus, we hypothesized that conditional ablation of Sox17 would lead to hyperproliferation of endometrial glands in mice. Contrary to our prediction, disruption of Sox17 in epithelial and stromal compartments led to inhibition of endometrial adenogenesis and a loss of reproductive capacity. Epithelium-specific Sox17 disruption resulted in normal adenogenesis although reproductive capacity remained impaired. These findings suggest that non-epithelial, Sox17-positive cells are necessary for adenogenesis and that glands require Sox17 to properly function. To our knowledge, these findings are the first to implicate Sox17 in endometrial gland formation and reproductive success. The data presented herein underscore the importance of studying Sox17 in uterine homeostasis and function. •Multi-compartment uterine Sox17 deletion impairs normal uterine function.•Non-epithelial, Sox17-positive stromal cells are necessary for gland formation.•Mice with Sox17-negative glandular cells are unable to reproduce.

Development

Adenogenesis

Mouse model

Uterus

Endometrial glands

Sox17

Details

Title: Subtitle: Conditional deletion of Sox17 reveals complex effects on uterine adenogenesis and function
Creators: Amy Guimarães-Young - Department of Anatomy and Cell Biology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Traci Neff - Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Adam J Dupuy - Department of Anatomy and Cell Biology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Michael J Goodheart - Department of Anatomy and Cell Biology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Developmental Biology, Vol.414(2), pp.219-227
DOI: 10.1016/j.ydbio.2016.04.010
PMID: 27102016
PMCID: PMC5521196
NLM abbreviation: Dev Biol
ISSN: 0012-1606
eISSN: 1095-564X
Publisher: Elsevier Inc
Language: English
Date published: 06/15/2016
Academic Unit: Radiology; Anatomy and Cell Biology; Pathology; Obstetrics and Gynecology; Internal Medicine
Record Identifier: 9983931566202771

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