Journal article
Conditional deletion of insulin receptor in thyrocytes does not affect thyroid structure and function
Endocrine journal, Vol.58(11), pp.1013-1019
2011
DOI: 10.1507/endocrj.EJ11-0007
PMCID: PMC3471790
PMID: 21908931
Abstract
Thyroid-stimulating hormone (TSH) is the primary regulator of thyroid growth and function acting via cyclic AMP signaling cascades. In cultured thyrocytes, insulin and/or insulin-like growth factor-1 (IGF-1) are required for mediating thyrocyte proliferation in concert with TSH. To determine the role of insulin signaling in thyroid, growth in vivo, mice with thyrocyte-selective ablation of the insulin receptor (IR) were generated by crossing mice homozygous for a floxed IR allele with transgenic mice in which thyrocyte-specific expression of Cre recombinase was driven by the human thyroid peroxidase (TPO) gene promoter. Immunohistochemistry and Western blot analysis confirmed near complete loss of IR expression in the thyroid of thyrocyte IR knockout mice. These mice are viable and have no obvious thyroid dysfunction and macro- and microscopic thyroid morphology was normal. Thus, insulin signaling in thyrocytes does not play an essential role in the architecture and function of the thyroid in vivo.
Details
- Title: Subtitle
- Conditional deletion of insulin receptor in thyrocytes does not affect thyroid structure and function
- Creators
- Sangmi Ock - Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, KoreaSeok Hong Lee - Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, KoreaJihyun Ahn - Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, KoreaTae Jin Lee - Department of Pathology, College of Medicine, Chung-Ang University, Seoul, KoreaChung-Hyun Cho - Department of Pharmacology, College of Medicine, Seoul National University, Seoul, KoreaE. Dale Abel - Division of Endocrinology, Metabolism and Diabetes, Program in Molecular Medicine, University of Utah School of Medicine, Salt Lake City, USAShioko Kimura - Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, USAJaetaek Kim - Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, Korea
- Resource Type
- Journal article
- Publication Details
- Endocrine journal, Vol.58(11), pp.1013-1019
- Publisher
- 一般社団法人 日本内分泌学会
- DOI
- 10.1507/endocrj.EJ11-0007
- PMID
- 21908931
- PMCID
- PMC3471790
- ISSN
- 0918-8959
- eISSN
- 1348-4540
- Language
- English
- Date published
- 2011
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984024506402771
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