Journal article
Confirmation of extended-spectrum β-lactamase-producing Serratia marcescens: preliminary report from Taiwan
Diagnostic microbiology and infectious disease, Vol.45(4), pp.221-224
2003
DOI: 10.1016/S0732-8893(02)00539-4
PMID: 12729989
Abstract
Although Serratia marcescens is a common cause of nosocomial infection in Taiwan, strains producing extended-spectrum β-lactamases (ESBLs) are rare. We detected four clinical isolates of S. marcescens from Taiwan that exhibited resistance to cefotaxime (MICs, > 256 μg/ml) and cefepime (MICs, ≥ 32 μg/ml), but were susceptible to imipenem and meropenem. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4), which was further identified as CTX-M-3 by polymerase chain reaction and gene sequencing. An AmpC-like enzyme (pI 8.8) was not transferable. All four isolates had significant MIC reductions of ≥3 log 2 dilutions for cefepime in the presence of clavulanic acid, compatible with the presence of an ESBL (CTX-M-3). Clavulanate did not significantly reduce the cefotaxime MIC for one isolate that may co-produce high-level AmpC β-lactamase (pI 8.8). Since high-level AmpC expression has minimal effect on the activity of cefepime, isolates co-producing AmpC β-lactamase may be recognized as additional ESBL producers by using cefepime as an ESBL screening agent.
Details
- Title: Subtitle
- Confirmation of extended-spectrum β-lactamase-producing Serratia marcescens: preliminary report from Taiwan
- Creators
- Wen-Liang Yu - Section of Infectious Diseases, Department of Medicine, China Medical College Hospital, Yuh Der Road, Taichung City, TaiwanLii-Tzu Wu - Department of Microbiology, Institute of Medical Science, China Medical College, Taichung City, TaiwanMichael A Pfaller - The University of Iowa College of Medicine, Iowa City, Iowa, USAPatricia L Winokur - The University of Iowa College of Medicine, Iowa City, Iowa, USARonald N Jones - The JONES Group/JMI Laboratories, North Liberty, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.45(4), pp.221-224
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0732-8893(02)00539-4
- PMID
- 12729989
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 2003
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Medicine Administration; Internal Medicine
- Record Identifier
- 9984094654802771
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