Journal article
Confocal Imaging of Microglial Cell Dynamics in Hippocampal Slice Cultures
Methods (San Diego, Calif.), Vol.18(2), pp.222-230
06/1999
DOI: 10.1006/meth.1999.0775
PMID: 10356354
Abstract
Methods are described for imaging the cellular dynamics of microglia in live mammalian brain slice cultures. Brain slices prepared from developing rat hippocampus are cultured for up to 2 weeks by the roller tube or static filter culture technique, stained with one or more fluorescent dyes, and imaged by scanning laser confocal microscopy. One of several cell type-specific or nonspecific fluorescent dyes can be used independently or in combination to label cells in live brain tissues. The fluorescently conjugated plant isolectin GSA-IB4 is useful for identifying microglia and for following their structure, movement, and proliferation. Live and dead neurons and glia can be distinguished using membrane-permeant and -impermeant fluorescent nucleic acid dyes. Nonspecific fluorescent lipids such as DiIC18 can be used as a vital stain to label populations of endocytic and phagocytic cells. Using multichannel confocal imaging, tissue slices that are single-, double-, or triple-labeled can be imaged in the living state in two or three spatial dimensions as well as in time. This provides a means for investigating the cell–cell interaction and dynamic behavior of microglia and other cell types in live brain tissues cultured under various physiological conditions.
Details
- Title: Subtitle
- Confocal Imaging of Microglial Cell Dynamics in Hippocampal Slice Cultures
- Creators
- Michael E DaileyMarc Waite
- Resource Type
- Journal article
- Publication Details
- Methods (San Diego, Calif.), Vol.18(2), pp.222-230
- Publisher
- Elsevier Inc
- DOI
- 10.1006/meth.1999.0775
- PMID
- 10356354
- ISSN
- 1046-2023
- eISSN
- 1095-9130
- Language
- English
- Date published
- 06/1999
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070316102771
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