Conformation-driven strategy for resilient and functional protein materials

Xuan Mu; John S K Yuan Jr; Jaewon Choi; Yixin Zhang; Peggy Cebe; Xiaocheng Jiang; Yu Shrike Zhang; David L Kaplan

doi:10.1073/pnas.2115523119

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Conformation-driven strategy for resilient and functional protein materials

Journal article

Open access

Peer reviewed

Conformation-driven strategy for resilient and functional protein materials

Xuan Mu, John S K Yuan Jr, Jaewon Choi, Yixin Zhang, Peggy Cebe, Xiaocheng Jiang, Yu Shrike Zhang and David L Kaplan

Proceedings of the National Academy of Sciences - PNAS, Vol.119(4), p.1

01/25/2022

DOI: 10.1073/pnas.2115523119

PMCID: PMC8795527

PMID: 35074913

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https://doi.org/10.1073/pnas.2115523119View

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Abstract

The exceptional elastic resilience of some protein materials underlies essential biomechanical functions with broad interest in biomedical fields. However, molecular design of elastic resilience is restricted to amino acid sequences of a handful of naturally occurring resilient proteins such as resilin and elastin. Here, we exploit non-resilin/elastin sequences that adopt kinetically stabilized, random coil-dominated conformations to achieve near-perfect resilience comparable with that of resilin and elastin. We also show a direct correlation between resilience and Raman-characterized protein conformations. Furthermore, we demonstrate that metastable conformation of proteins enables the construction of mechanically graded protein materials that exhibit spatially controlled conformations and resilience. These results offer insights into molecular mechanisms of protein elastomers and outline a general conformation-driven strategy for developing resilient and functional protein materials.

Amino Acid Sequence

Fibroins - chemistry

Models, Molecular

Protein Conformation

Proteins - chemistry

Spectrum Analysis

Structure-Activity Relationship

Details

Title: Subtitle: Conformation-driven strategy for resilient and functional protein materials
Creators: Xuan Mu - Tufts University
John S K Yuan Jr - Tufts University
Jaewon Choi - Tufts University
Yixin Zhang - Tufts University
Peggy Cebe - Tufts University
Xiaocheng Jiang - Tufts University
Yu Shrike Zhang - Brigham and Women's Hospital
David L Kaplan - Tufts University
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.119(4), p.1
DOI: 10.1073/pnas.2115523119
PMID: 35074913
PMCID: PMC8795527
ISSN: 0027-8424
eISSN: 1091-6490
Grant note: R21 EB030257 / NIBIB NIH HHS UG3 TR003274 / NCATS NIH HHS U01 EB014976 / NIBIB NIH HHS R01 GM134036 / NIGMS NIH HHS R01 HL153857 / NHLBI NIH HHS R00 CA201603 / NCI NIH HHS R21 EB025270 / NIBIB NIH HHS R01 EB028143 / NIBIB NIH HHS P41 EB027062 / NIBIB NIH HHS R21 EB026175 / NIBIB NIH HHS
Language: English
Date published: 01/25/2022
Academic Unit: Roy J. Carver Department of Biomedical Engineering
Record Identifier: 9984277266902771

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