Journal article
Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery
Bioconjugate chemistry, Vol.18(6), pp.2068-2076
11/2007
DOI: 10.1021/bc070116l
PMID: 17848077
Abstract
We report on the preparation and characterization of poly(D, L-lactide-co-glycolide) (PLGA) microparticles with surface-conjugated polyamidoamine (PAMAM) dendrimers of varying generations. The buffering capacity and zeta-potential of the PLGA PAMAM microparticles increased with increasing generation level of the PAMAM dendrimer conjugated. Conjugation of the PAMAM dendrimer to the surface of the PLGA microparticle removed generation-dependent cytotoxicity in HEK293 and COS7 cell lines. PLGA PAMAM pDNA microparticles displayed similar cytotoxicity profiles to unmodified PLGA pDNA microparticles in COS7 cells. A generation three PAMAM dendrimer conjugated to PLGA microparticles significantly increased transfection efficiencies in comparison to unmodified PLGA microparticles.
Details
- Title: Subtitle
- Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery
- Creators
- Xue-Qing Zhang - Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USAJanjira IntraAliasger K Salem
- Resource Type
- Journal article
- Publication Details
- Bioconjugate chemistry, Vol.18(6), pp.2068-2076
- DOI
- 10.1021/bc070116l
- PMID
- 17848077
- NLM abbreviation
- Bioconjug Chem
- ISSN
- 1043-1802
- eISSN
- 1520-4812
- Publisher
- United States
- Language
- English
- Date published
- 11/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983985965602771
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