Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

Ted S Acott; Michael P Fautsch; Weiming Mao; C Ross Ethier; Alex S Huang; Mary J Kelley; Mini Aga; Sanjoy K Bhattacharya; Terete Borras; Diane Bovenkamp; Uttio Roy Chowdhury; Abbot F Clark; Mohammed I Dibas; Yiqin Du; Michael H Elliott; Jennifer A Faralli; Haiyan Gong; Samuel Herberg; Murray A Johnstone; Paul L Kaufman; Kate E Keller; Ruth A Kelly; David Krizaj; Markus H Kuehn; Hoi Lam Li; Raquel Lieberman; Shan C Lin; Yutao Liu; Fiona S McDonnell; Colleen M McDowell; Gillian J McLellan; Philip Mzyk; Kayarat Saidas Nair; Darryl R Overby; Donna M Peters; VijayKrishna Raghunathan; Ponugoti Vasantha Rao; Gavin W Roddy; Najam A Sharif; Myoung Sup Shim; Yang Sun; Benjamin R Thomson; Carol B Toris; Colin E Willoughby; Hao F Zhang; Thomas F Freddo; Rudolf Fuchshofer; Kamisha R Hill; Alireza Karimi; Krishnakumar Kizhatil; Casey C Kopcyznski; Paloma Liton; Gaurang Patel; Michael Peng; Padmanabhan P Pattabiraman; Ganesh Prasanna; Ester Reina-Torres; E Griffen Samples; John R Samples; Cynthia L Steel; Clemens A Strohmaier; Preeti Subramanian; Chenna Kesavulu Sugali; Joseph van Batenburg-Sherwood; Cydney Wong; Hannah Youngblood; Gulab S Zode; Elizabeth White; W Daniel Stamer

doi:10.1167/iovs.65.14.32

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Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

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Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

Ted S Acott, Michael P Fautsch, Weiming Mao, C Ross Ethier, Alex S Huang, Mary J Kelley, Mini Aga, Sanjoy K Bhattacharya, Terete Borras, Diane Bovenkamp, …

Investigative ophthalmology & visual science, Vol.65(14), 32

12/02/2024

DOI: 10.1167/iovs.65.14.32

PMCID: PMC11708870

PMID: 39693082

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Abstract

Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.

Animals

Anterior Eye Segment

Aqueous Humor - metabolism

Aqueous Humor - physiology

Consensus

Glaucoma - diagnosis

Glaucoma - physiopathology

Humans

Intraocular Pressure - physiology

Organ Culture Techniques

Perfusion - methods

Trabecular Meshwork

Details

Title: Subtitle: Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches
Creators: Ted S Acott - Oregon Health & Science University
Michael P Fautsch - Mayo Clinic
Weiming Mao - Indiana University School of Medicine
C Ross Ethier - The Wallace H. Coulter Department of Biomedical Engineering
Alex S Huang - University of California San Diego
Mary J Kelley - Oregon Health & Science University
Mini Aga - Oregon Health & Science University
Sanjoy K Bhattacharya - University of Miami
Terete Borras - University of North Carolina at Chapel Hill
Diane Bovenkamp - BrightFocus Foundation
Uttio Roy Chowdhury - Mayo Clinic
Abbot F Clark - University of North Texas Health Science Center
Mohammed I Dibas - AbbVie
Yiqin Du - University of South Florida
Michael H Elliott - University of Oklahoma Health Sciences Center
Jennifer A Faralli - University of Wisconsin–Madison
Haiyan Gong - Boston University
Samuel Herberg - SUNY Upstate Medical University
Murray A Johnstone - University of Washington
Paul L Kaufman - University of Wisconsin–Madison
Kate E Keller - Oregon Health & Science University
Ruth A Kelly - Duke University
David Krizaj - University of Utah
Markus H Kuehn - University of Iowa
Hoi Lam Li - Boston University
Raquel Lieberman - Georgia Institute of Technology
Shan C Lin - Glaucoma Research Foundation
Yutao Liu - Augusta University
Fiona S McDonnell - Eye Institute of Utah
Colleen M McDowell - University of Wisconsin–Madison
Gillian J McLellan - University of Wisconsin–Madison
Philip Mzyk - Duke University
Kayarat Saidas Nair - University of California, San Francisco
Darryl R Overby - Imperial College London
Donna M Peters - University of Wisconsin–Madison
VijayKrishna Raghunathan - Novartis Institutes for BioMedical Research
Ponugoti Vasantha Rao - Duke University
Gavin W Roddy - Mayo Clinic
Najam A Sharif - Santen
Myoung Sup Shim - Duke University
Yang Sun - Stanford University
Benjamin R Thomson - Northwestern University
Carol B Toris - The Ohio State University Wexner Medical Center
Colin E Willoughby - University of Ulster
Hao F Zhang - Northwestern University
Thomas F Freddo - MCPHS University
Rudolf Fuchshofer - University of Regensburg
Kamisha R Hill - Georgia Institute of Technology
Alireza Karimi - Oregon Health & Science University
Krishnakumar Kizhatil - The Ohio State University
Casey C Kopcyznski - Aerie Pharmaceuticals
Paloma Liton - Duke University
Gaurang Patel - University of North Texas Health Science Center
Michael Peng - Indiana University School of Medicine
Padmanabhan P Pattabiraman - Indiana University School of Medicine
Ganesh Prasanna - Novartis
Ester Reina-Torres - Imperial College London
E Griffen Samples - Glaucoma Foundation
John R Samples - Washington State University Spokane
Cynthia L Steel
Clemens A Strohmaier - Johannes Kepler University of Linz
Preeti Subramanian - BrightFocus Foundation
Chenna Kesavulu Sugali - Indiana University School of Medicine
Joseph van Batenburg-Sherwood - Imperial College London
Cydney Wong - The Wallace H. Coulter Department of Biomedical Engineering
Hannah Youngblood - Georgia Institute of Technology
Gulab S Zode - University of California, Irvine
Elizabeth White - Oregon Health & Science University
W Daniel Stamer - Duke University
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.65(14), 32
DOI: 10.1167/iovs.65.14.32
PMID: 39693082
PMCID: PMC11708870
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 1552-5783
eISSN: 1552-5783
Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Grant note: National Eye Institute: EY026529, EY031631, EY033961, EY023242, EY032960, EY028671, EY014800, EY027920, EY031817, EY002423, EY021205, EY026885, EY033600, EY034096, EY022634, EY025643, EY026962, EY031700, EY033929, EY036011 BrightFocus FoundationResearch to Prevent BlindnessAerie PharmaceuticalsGlaukos CorporationGlaucoma Research FoundationWestern Glaucoma FoundationLewis Rudin Glaucoma PrizeAllergan by AbbVie
Supported by grants from the National Eye Institute, National EY026529 to CMM; EY031631 to YL; EY033961 to YL; EY023242 to YL; EY032960 to YL; EY028671 to YL; EY014800 to DK; EY027920 to DK; EY031817 to DK; EY002423 to DK; EY021205 to RLL; EY026885 to PL; EY033600 to PL; EY034096 to SH; EY022634 to HG; EY025643 to YD; EY026962 to WM; EY031700 to WM; EY033929 to WM; EY036011 to AK) ; The BrightFocus Foundation; Research to Prevent Blindness; Aerie Pharmaceuticals; Glaukos Corporation; Glaucoma Research Foundation; Western Glaucoma Foundation; Lewis Rudin Glaucoma Prize to MJK) ; and Allergan by AbbVie.
Language: English
Date published: 12/02/2024
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9984758266202771

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