Journal article
Consensus recommendations for trabecular meshwork cell isolation, characterization and culture
Experimental eye research, Vol.171, pp.164-173
06/01/2018
DOI: 10.1016/j.exer.2018.03.001
PMCID: PMC6042513
PMID: 29526795
Abstract
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
•Comprehensive review of techniques to culture TM cells are presented.•Helpful tips for culturing TM cells are described.•First set of consensus standards for TM cell research is defined.
Details
- Title: Subtitle
- Consensus recommendations for trabecular meshwork cell isolation, characterization and culture
- Creators
- Kate E. Keller - Oregon Health and Science University, United StatesSanjoy K. Bhattacharya - University of Miami, United StatesTheresa Borrás - University of North Carolina at Chapel HillThomas M. Brunner - Glaucoma Research Foundation, United StatesSunee Chansangpetch - University of California, San Francisco, United StatesAbbott F. Clark - University of North Texas Health Science CenterW. Michael Dismuke - Aerie Pharmaceuticals, United StatesYiqin Du - University of Pittsburgh, United StatesMichael H. Elliott - University of Oklahoma, United StatesC. Ross Ethier - The Wallace H. Coulter Department of Biomedical EngineeringJennifer A. Faralli - University of Wisconsin, United StatesThomas F. Freddo - Massachusetts College of Pharmacy and Health Sciences, United StatesRudolf Fuchshofer - University of Regensburg, GermanyMichael Giovingo - John H. Stroger, Jr. Hospital of Cook County, United StatesHaiyan Gong - Boston UniversityPedro Gonzalez - Duke UniversityAlex Huang - University of California, Los Angeles, United StatesMurray A. Johnstone - University of Washington, United StatesPaul L. Kaufman - University of Wisconsin, United StatesPaul A. Knepper - University of Illinois at Chicago, United StatesMary J. Kelley - Oregon Health and Science University, United StatesCasey C. Kopczynski - Aerie Pharmaceuticals, United StatesJohn G. Kuchtey - Vanderbilt University, United StatesRachel W. Kuchtey - Vanderbilt University, United StatesMarkus H. Kuehn - University of Iowa, Ophthalmology and Visual SciencesRaquel L. Lieberman - Georgia Institute of Technology, United StatesShan C. Lin - University of California, San Francisco, United StatesPaloma Liton - Duke University, United StatesYutao Liu - Augusta University, United StatesElke Lütjen-Drecoll - Friedrich-Alexander-Universität Erlangen-NürnbergWeiming Mao - University of North Texas Health Sciences Center, United StatesMarisse Masis-Solano - University of California, San Francisco, United StatesFiona McDonnell - Duke University, United StatesColleen M. McDowell - University of Wisconsin–MadisonDarryl R. Overby - Imperial College, London, United KingdomPadmanabhan P. Pattabiraman - Case Western Reserve University, United StatesVijay K. Raghunathan - University of Houston, United StatesP. Vasanth Rao - Duke UniversityDouglas J. Rhee - Case Western Reserve University, United StatesUttio Roy Chowdhury - Mayo Clinic, United StatesPaul Russell - University of California, Davis, United StatesJohn R. Samples - Western Glaucoma Foundation, United StatesDonald Schwartz - EyeSonix, United StatesEvan B. Stubbs - Loyola University, Chicago, United StatesErnst R. Tamm - University of RegensburgJames C. Tan - University of Southern California, United StatesCarol B. Toris - Case Western Reserve University, United StatesKaren Y. Torrejon - Glauconix, United StatesJanice A. Vranka - Oregon Health and Science University, United StatesMary K. Wirtz - Oregon Health and Science University, United StatesThomas Yorio - University of North Texas Health Sciences Center, United StatesJie Zhang - University of California, Los Angeles, United StatesGulab S. Zode - University of North Texas Health Sciences Center, United StatesMichael P. Fautsch - Department of Ophthalmology, Mayo Clinic, 200 1st St SW, Rochester, MN 55905, United StatesDonna M. Peters - Department of Pathology & Laboratory Medicine, University of Wisconsin, 1300 University Ave, Madison, WI 53706, United StatesTed S. Acott - Department of Ophthalmology, Department of Biochemistry & Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, United StatesW. Daniel Stamer - Department of Ophthalmology, Duke University, DUMC 3802, Durham, NC 27705, United States
- Resource Type
- Journal article
- Publication Details
- Experimental eye research, Vol.171, pp.164-173
- DOI
- 10.1016/j.exer.2018.03.001
- PMID
- 29526795
- PMCID
- PMC6042513
- NLM abbreviation
- Exp Eye Res
- ISSN
- 0014-4835
- eISSN
- 1096-0007
- Publisher
- Elsevier Ltd
- Grant note
- name: Griff Samples; name: Thelma De Souza; name: Aerie Pharmaceuticals; DOI: 10.13039/100007489, name: Bausch and Lomb; name: Alcon/Novartis; name: Glaukos; name: Western Glaucoma Foundation
- Language
- English
- Date published
- 06/01/2018
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9983979983102771
Metrics
27 Record Views