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Conservation of nonpeptide antigen recognition by rhesus monkey V gamma 2V delta 2 T cells
Journal article   Peer reviewed

Conservation of nonpeptide antigen recognition by rhesus monkey V gamma 2V delta 2 T cells

Hong Wang, Hoi K Lee, Jack F Bukowski, Hongmin Li, Roy A Mariuzza, Zheng W Chen, Ki-Hoan Nam and Craig T Morita
The Journal of immunology (1950), Vol.170(7), pp.3696-3706
04/01/2003
DOI: 10.4049/jimmunol.170.7.3696
PMID: 12646635

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Abstract

We have previously found that monkey Vgamma2Vdelta2(+) T cells mount adaptive immune responses in response to Mycobacterium bovis bacillus Calmette-Guérin infections. We have now analyzed rhesus monkey gammadelta T cell responses to nonpeptide Ags and superantigens. Like human Vgamma2Vdelta2(+) T cells, rhesus monkey gammadelta T cells are stimulated when exposed to prenyl pyrophosphate, bisphosphonate, and alkylamine Ags. Responsiveness was limited to gammadelta T cells expressing Vgamma2Vdelta2 TCRs. Rhesus monkey Vgamma2Vdelta2(+) T cells also responded to the superantigen, staphyloccocal enterotoxin A. Sequencing of the rhesus monkey Vgamma2Vdelta2 TCR revealed a strong sequence homology to human Vgamma2Vdelta2 TCR that preserves important sequence motifs. Moreover, chimeric TCRs that pair human Vgamma2 with monkey Vdelta2 and monkey Vgamma2 with human Vdelta2 retain reactivity to nonpeptide Ags and B cell lymphomas. A molecular model of the rhesus monkey Vgamma2Vdelta2 TCR has a basic region in the complementarity-determining region 3 binding groove that is similar to that seen in the human Vgamma2Vdelta2 TCR and preserves the topology of the complementarity-determining region loops. Thus, recognition of nonpeptide prenyl pyrophosphate, bisphosphonate, and alkylamine Ags is conserved in primates suggesting that primates can provide an animal model for human gammadelta T cell Ag responses.
 Recombinant Fusion Proteins - immunology T-Lymphocyte Subsets - immunology Diphosphates - pharmacology Organophosphorus Compounds - immunology Receptors, Antigen, T-Cell, gamma-delta - blood Humans Butylamines - pharmacology Recombinant Fusion Proteins - analysis Molecular Sequence Data Receptors, Antigen, T-Cell, gamma-delta - genetics Macaca mulatta Organophosphorus Compounds - metabolism Recombinant Fusion Proteins - metabolism Lymphocyte Activation - immunology Transfection Organophosphorus Compounds - pharmacology Clone Cells Conserved Sequence - immunology Epitopes, T-Lymphocyte - immunology Tumor Cells, Cultured Infant, Newborn Amino Acid Sequence Fetal Blood - immunology Butylamines - immunology Epitopes, T-Lymphocyte - analysis Receptors, Antigen, T-Cell, gamma-delta - biosynthesis Antigens, Bacterial - pharmacology Antigen Presentation - immunology Fetal Blood - metabolism Receptors, Antigen, T-Cell, gamma-delta - isolation & purification Butylamines - metabolism Sequence Homology, Amino Acid Animals Lymphocyte Activation - drug effects T-Lymphocyte Subsets - metabolism Hemiterpenes Superantigens - pharmacology Antigens, Bacterial - metabolism Epitopes, T-Lymphocyte - metabolism

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