Conservation of the biologically active portions of staphylococcal enterotoxins C1 and C2

Gregory A Bohach; Patrick M Schlievert

doi:10.1128/IAI.57.7.2249-2252.1989

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Conservation of the biologically active portions of staphylococcal enterotoxins C1 and C2

Journal article

Open access

Peer reviewed

Conservation of the biologically active portions of staphylococcal enterotoxins C1 and C2

Gregory A Bohach and Patrick M Schlievert

Infection and immunity, Vol.57(7), pp.2249-2252

07/1989

DOI: 10.1128/IAI.57.7.2249-2252.1989

PMCID: PMC313868

PMID: 2543637

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Abstract

We determined the primary sequence of staphylococcal enterotoxin (SE) C2 by sequencing its cloned structural gene, entC2. The entC2 structural gene contains an 801-base-pair open reading frame which encodes a 266-amino-acid precursor with a molecular weight of 30,608. Mature SE C2, produced by removal of the signal peptide, contains 239 amino acids with a molecular weight of 27,531. A sequence comparison between SE C2 and SE C1 showed that the 167 carboxyl amino acids in both toxins were 100% conserved. In contrast, the 72 N-terminal residues were 10% divergent. This provides additional evidence that carboxyl regions of staphylococcal and streptococcal pyrogenic toxins determine shared biological activities and cross-reactive epitopes.

Staphylococcus aureus - genetics

Amino Acid Sequence

Genes, Bacterial

Enterotoxins - toxicity

Staphylococcus aureus - physiology

Bacterial Proteins - genetics

Molecular Sequence Data

Antigens, Bacterial - isolation & purification

Enterotoxins - isolation & purification

Antigens, Bacterial - genetics

Sequence Homology, Nucleic Acid

Enterotoxins - genetics

Bacterial Proteins - toxicity

Base Sequence

Bacterial Proteins - isolation & purification

Details

Title: Subtitle: Conservation of the biologically active portions of staphylococcal enterotoxins C1 and C2
Creators: Gregory A Bohach - Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455
Patrick M Schlievert
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.57(7), pp.2249-2252
Publisher: United States
DOI: 10.1128/IAI.57.7.2249-2252.1989
PMID: 2543637
PMCID: PMC313868
ISSN: 0019-9567
eISSN: 1098-5522
Grant note: HL36611 / NHLBI NIH HHS
Language: English
Date published: 07/1989
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001154902771

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