Journal article
Conserved Requirement of Lim1 Function for Cell Movements during Gastrulation
Developmental cell, Vol.4(1), pp.83-94
2003
DOI: 10.1016/S1534-5807(02)00398-2
PMID: 12530965
Abstract
To investigate
Lim1 function during gastrulation, we used transcript depletion through DEED antisense oligonucleotides in
Xenopus and cell transplantation in mice.
Xenopus embryos depleted of
Lim1 lack anterior head structures and fail to form a proper axis as a result of a failure of gastrulation movements, even though mesodermal cell identities are specified. Similar disruption of cell movements in the mesoderm is also observed in
Lim1
−/−
mice. Paraxial protocadherin (PAPC) expression is lost in the nascent mesoderm of
Lim1
−/−
mouse embryos and in the organizer of
Lim1-depleted
Xenopus embryos; the latter can be rescued to a considerable extent by supplying PAPC exogenously. We conclude that a primary function of
Lim1 in the early embryo is to enable proper cell movements during gastrulation.
Details
- Title: Subtitle
- Conserved Requirement of Lim1 Function for Cell Movements during Gastrulation
- Creators
- Neil A Hukriede - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 USATania E Tsang - Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville NSW 2145, AustraliaRaymond Habas - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 USAPoh-Lynn Khoo - Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville NSW 2145, AustraliaKirsten Steiner - Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville NSW 2145, AustraliaDaniel L Weeks - Department of Biochemistry, University of Iowa, Iowa City, IA 52242 USAPatrick P.L Tam - Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville NSW 2145, AustraliaIgor B Dawid - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 USA
- Resource Type
- Journal article
- Publication Details
- Developmental cell, Vol.4(1), pp.83-94
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S1534-5807(02)00398-2
- PMID
- 12530965
- ISSN
- 1534-5807
- eISSN
- 1878-1551
- Language
- English
- Date published
- 2003
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology
- Record Identifier
- 9984024510602771
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