Journal article
Constitutive Activation of Transcription Factor AP-2 Is Associated with Decreased MnSOD Expression in Transformed Human Lung Fibroblasts
Antioxidants & redox signaling, Vol.3(3), pp.387-395
06/2001
DOI: 10.1089/15230860152409031
PMID: 11491651
Abstract
Activator protein-2 (AP-2) is a transcription factor with transactivating and transrepressing potential in different promoter contexts. AP-2 contains seven cysteines, and its in vitro DNA binding activity is redox-sensitive. Superoxide dismutase-2 (SOD2), which encodes the antioxidant enzyme manganese superoxide dismutase (MnSOD), is a putative tumor suppressor gene whose loss of expression is associated with the malignant phenotype. SOD2 promoter mutations that generate new AP-2 sites are associated with loss of MnSOD expression in cancer cells. In the current study, we have identified an inverse expression pattern between AP-2 and MnSOD in normal versus transformed human cells. MRC5 cells are a normal human lung fibroblast cell strain that is mortal and senesces after a certain number of passages in vitro. MRC5-VA is a simian virus transformed variant of MRC5. We determined the levels of expression of MnSOD and AP-2 in these two cell types at the levels of mRNA, protein, and activity. Our results indicated that MnSOD expression was significantly decreased in MRC5-VA cells compared with MRC5 cells at each level of investigation, whereas AP-2 showed an opposing pattern of expression and DNA binding activity. These results suggest that AP-2 may participate in the mechanism(s) underlying decreased expression of SOD2 in transformed cells.
Details
- Title: Subtitle
- Constitutive Activation of Transcription Factor AP-2 Is Associated with Decreased MnSOD Expression in Transformed Human Lung Fibroblasts
- Creators
- Chunhong ZhuYuanhui HuangChristine J WeydertLarry W OberleyFrederick E Domann
- Resource Type
- Journal article
- Publication Details
- Antioxidants & redox signaling, Vol.3(3), pp.387-395
- DOI
- 10.1089/15230860152409031
- PMID
- 11491651
- ISSN
- 1523-0864
- eISSN
- 1557-7716
- Language
- English
- Date published
- 06/2001
- Academic Unit
- Pathology; Surgery; Radiation Oncology
- Record Identifier
- 9984047978602771
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