Journal article
Constitutive activity of the lutropin receptor and its allosteric modulation by receptor heterodimerization
Methods in enzymology, Vol.484(C), pp.231-252
2010
DOI: 10.1016/B978-0-12-381298-8.00012-5
PMID: 21036235
Abstract
The lutropin receptor (LHR) is a G protein-coupled receptor (GPCR) that mediates the actions of pituitary LH in males and females and that of placental hCG in pregnant women and, therefore, plays an essential role in reproductive physiology. Mutations of the lhcgr gene that result in constitutive activation of the LHR have been shown to be causative of gonadotropin-independent precocious puberty in young boys. Studies on constitutively active mutants (CAMs) of the LHR have been extremely informative in elucidating the roles of the LHR in reproductive physiology as well as in understanding the molecular basis underlying activation of this GPCR. The constitutive activities of hLHR CAMs can be attenuated by introducing mutations into the CAMs that stabilize the resting state of the hLHR or by coexpressing the hLHR CAMs with an hLHR mutant that is stabilized in the resting state, allowing the two forms of the hLHR to heterodimerize. This chapter describes the experimental methods and strategies underlying studies of hLHR CAMs.
Details
- Title: Subtitle
- Constitutive activity of the lutropin receptor and its allosteric modulation by receptor heterodimerization
- Creators
- Deborah L Segaloff - Department of Physiology and Biophysics, The University of Iowa Roy J. and Lucille A. Carver College ofMedicine, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Methods in enzymology, Vol.484(C), pp.231-252
- DOI
- 10.1016/B978-0-12-381298-8.00012-5
- PMID
- 21036235
- ISSN
- 0076-6879
- eISSN
- 1557-7988
- Grant note
- DK068614 / NIDDK NIH HHS HD22196 / NICHD NIH HHS
- Language
- English
- Date published
- 2010
- Academic Unit
- Molecular Physiology and Biophysics; Obstetrics and Gynecology
- Record Identifier
- 9984083216702771
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