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Constitutive expression of Epstein-Barr virus-encoded RNAs and nuclear antigen during latency and after induction of Epstein-Barr virus replication
Journal article   Open access   Peer reviewed

Constitutive expression of Epstein-Barr virus-encoded RNAs and nuclear antigen during latency and after induction of Epstein-Barr virus replication

R Weigel, D. K Fischer, L Heston and G Miller
Journal of virology, Vol.53(1), pp.254-259
1985
DOI: 10.1128/jvi.53.1.254-259.1985
PMCID: PMC255024
PMID: 2981344
url
https://europepmc.org/articles/pmc255024View
Published (Version of record) Open Access

Abstract

We examined the fate of two major products of latency as Epstein-Barr virus was induced to replicate. We studied a superinducible clone of HR-1 cells in the presence and absence of induction by phorbol ester, and we analyzed the X50-7 line with and without superinfection by an HR-1 viral variant which disrupts latency. The two methods of induction yielded qualitatively similar results. After induction, there was abundant synthesis of viral transcripts, amplification of viral DNA, and the appearance of many new viral polypeptides. Nonetheless, there were no changes in the cytoplasmic abundance of Epstein-Barr virus-encoded RNAs and no alteration in the level of Epstein-Barr virus nuclear antigen mRNA or polypeptide. Thus, under conditions in which numerous other Epstein-Barr virus gene products are activated, the two major latent gene products are expressed at a constitutive level. Expression of Epstein-Barr virus-encoded RNAs and nuclear antigen must therefore be regulated in a manner completely different from expression of replicative functions.
 Microbiology Virology Biological and medical sciences Fundamental and applied biological sciences. Psychology Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains

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