Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection

Jodie S Haring; Xuefang Jing; Julie Bollenbacher-Reilley; Hai-Hui Xue; Warren J Leonard; John T Harty

doi:10.4049/jimmunol.180.5.2855

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Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection

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Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection

Jodie S Haring, Xuefang Jing, Julie Bollenbacher-Reilley, Hai-Hui Xue, Warren J Leonard and John T Harty

The Journal of immunology (1950), Vol.180(5), pp.2855-2862

03/01/2008

DOI: 10.4049/jimmunol.180.5.2855

PMID: 18292507

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Abstract

Expression of IL-7Ralpha (CD127) has been suggested as a major determinant in the survival of memory T cell precursors. We investigated whether constitutive expression of IL-7Ralpha on T cells increased expansion and/or decreased contraction of endogenous Ag-specific CD4 and CD8 T cells following infection with Listeria monocytogenes. The results indicate that constitutive expression of IL-7Ralpha alone was not enough to impart an expansion or survival advantage to CD8 T cells responding to infection, and did not increase memory CD8 T cell numbers over those observed in wild-type controls. Constitutive expression of IL-7Ralpha did allow for slightly prolonged expansion of Ag-specific CD4 T cells; however, it did not alter the contraction phase or protect against the waning of memory T cell numbers at later times after infection. Memory CD4 and CD8 T cells generated in IL-7Ralpha transgenic mice expanded similarly to wild-type T cells after secondary infection, and immunized IL-7Ralpha transgenic mice were fully protected against lethal bacterial challenge demonstrating that constitutive expression of IL-7Ralpha does not impair, or markedly improve memory/secondary effector T cell function. These results indicate that expression of IL-7Ralpha alone does not support increased survival of effector Ag-specific CD4 or CD8 T cells into the memory phase following bacterial infection.

CD8-Positive T-Lymphocytes - cytology

Cell Proliferation

Cell Death - immunology

Cell Survival - genetics

Epitopes, T-Lymphocyte - genetics

Listeria monocytogenes - immunology

CD4-Positive T-Lymphocytes - immunology

Lymphocyte Activation - genetics

Receptors, Interleukin-7 - genetics

Cell Death - genetics

Listeriosis - immunology

CD8-Positive T-Lymphocytes - metabolism

Growth Inhibitors - biosynthesis

Epitopes, T-Lymphocyte - immunology

Growth Inhibitors - genetics

Immunologic Memory - genetics

Listeriosis - genetics

CD4-Positive T-Lymphocytes - cytology

Mice, Inbred C57BL

CD4-Positive T-Lymphocytes - metabolism

Mice, Transgenic

Cell Survival - immunology

Receptors, Interleukin-7 - physiology

Animals

Listeriosis - pathology

Growth Inhibitors - physiology

Lymphocyte Count

Mice

Receptors, Interleukin-7 - biosynthesis

CD8-Positive T-Lymphocytes - immunology

Details

Title: Subtitle: Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection
Creators: Jodie S Haring - Department of Microbiology, Carver School of Medicine, University of Iowa, Iowa City, IA 52242, USA
Xuefang Jing
Julie Bollenbacher-Reilley
Hai-Hui Xue
Warren J Leonard
John T Harty
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.180(5), pp.2855-2862
Publisher: United States
DOI: 10.4049/jimmunol.180.5.2855
PMID: 18292507
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AI42767 / NIAID NIH HHS AI46653 / NIAID NIH HHS AI50073 / NIAID NIH HHS
Language: English
Date published: 03/01/2008
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984046928802771

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