Journal article
Construction and partial characterization of two recombinant cDNA clones for procollagen from chicken cartilage
Nucleic acids research, Vol.10(4), pp.1175-1192
1982
DOI: 10.1093/nar/10.4.1175
PMID: 6280134
Abstract
Type II procollagen mRNA has been partially purified from embryonic chick sternal cartilage by guanidine hydrochloride extraction, sucrose gradient sedimentation and Sepharose 4B chromatography. Double stranded cDNA was synthesized using AMV reverse transcriptase and E. coli DNA polymerase I, tailed using terminal transferase and inserted into the Pst I site of pBR322. Two putative type II procollagen cDNA clones have been characterized. Both plasmids hybridize to 2 sternal RNA species, a major species of 5.3 kb and a minor species of 7 kb. These RNAs are present in total RNA from sterna and differentiated limb bud cultures but are not detected in RNA from stage 24 limb bud which has not yet differentiated to cartilage or in RNA from calvaria. The time of appearance of these RNAs during the differentiation of limb mesenchyme in culture parallels the appearance of translatable type II procollagen mRNA.
Details
- Title: Subtitle
- Construction and partial characterization of two recombinant cDNA clones for procollagen from chicken cartilage
- Creators
- Eero VuorioLinda SandellDean KravisVal C SheffieldTuula VuorioAlbert DorfmanWilliam B Upholt
- Resource Type
- Journal article
- Publication Details
- Nucleic acids research, Vol.10(4), pp.1175-1192
- DOI
- 10.1093/nar/10.4.1175
- PMID
- 6280134
- NLM abbreviation
- Nucleic Acids Res
- ISSN
- 0305-1048
- eISSN
- 1362-4962
- Language
- English
- Date published
- 1982
- Academic Unit
- Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics; Ophthalmology and Visual Sciences
- Record Identifier
- 9984065498002771
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