Journal article
Construction and properties of a recombinant herpes simplex virus 1 lacking both S-component origins of DNA synthesis
Journal of virology, Vol.67(4), pp.2123-2132
04/1993
DOI: 10.1128/JVI.67.4.2123-2132.1993
PMCID: PMC240309
PMID: 8383234
Abstract
The herpes simplex virus 1 (HSV-1) genome contains three origins of DNA synthesis (Ori) utilized by viral DNA synthesis proteins. One sequence (OriI) maps in the L component, whereas two sequences (OriS) map in the S component. We report the construction of a recombinant virus, R7711, from which both OriS sequences have been deleted, and show that the OriS sequences are not essential for the replication of HSV-1 in cultured cells. In addition to the deletions of OriS in R7711, the alpha 47 gene and the 5' untranscribed and transcribed noncoding regions of the U(S)11 gene were deleted, one of the alpha 4 promoter-regulatory regions was replaced with the simian virus 40 promoter, and the alpha 22 promoter was substituted with the alpha 27 promoter. The total amount of viral DNA synthesized in Vero cells infected with the OriS-negative (OriS-) virus was approximately that seen in cells infected with the OriS-positive virus. However, cells infected with the OriS- virus accumulated viral DNA more slowly than those infected with the wild-type virus during the first few hours after the onset of DNA synthesis. In single-step growth experiments, the yield of OriS- progeny virus was reduced at most fourfold. Although a single OriS (R. Longnecker and B. Roizman, J. Virol. 58:583-591, 1986) and the single OriL (M. Polvino-Bodnar, P. K. Orberg, and P. A. Schaffer, J. Virol. 61:3528-3535, 1987) have been shown to be dispensable, this is the first indication that both copies of OriS are dispensable and that one copy of an Ori sequence may suffice for the replication of HSV-1.
Details
- Title: Subtitle
- Construction and properties of a recombinant herpes simplex virus 1 lacking both S-component origins of DNA synthesis
- Creators
- Kazuhiko Igarashi - Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Illinois 60637Randall FawlRichard J RollerBernard Roizman
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.67(4), pp.2123-2132
- Publisher
- United States
- DOI
- 10.1128/JVI.67.4.2123-2132.1993
- PMID
- 8383234
- PMCID
- PMC240309
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Grant note
- AI124009 / NIAID NIH HHS CA47451 / NCI NIH HHS
- Language
- English
- Date published
- 04/1993
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001108602771
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