Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21

John H Greinwald Jr; Daryl A Scott; Jacquie R Marietta; Rivka Carmi; Jose Manaligod; Arabandi Ramesh; Ross I S Zbar; Michelle L Kraft; Khalil Elbedour; Yael Yairi; Maurice Musy; Anne B Skvorak; Guy Van Camp; C R Srikumari Srisailapathy; Michael Lovett; Cynthia C Morton; Val C Sheffield; Richard J H Smith

doi:10.1101/gr.7.9.879

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Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21

Journal article

Open access

Peer reviewed

Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21

John H Greinwald Jr, Daryl A Scott, Jacquie R Marietta, Rivka Carmi, Jose Manaligod, Arabandi Ramesh, Ross I S Zbar, Michelle L Kraft, Khalil Elbedour, Yael Yairi, …

Genome research, Vol.7(9), pp.879-886

09/1997

DOI: 10.1101/gr.7.9.879

PMID: 9314493

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Abstract

DFNB7 and DFNB11, two loci for autosomal recessive nonsyndromic hearing loss (ARNSHL), have been mapped to chromosome 9q13-21 in separate consanguineous families. Using a radiation hybrid map, we have determined the correct marker order in the DFNB7/11 region and have demonstrated that the DFNB11 locus resides within a redefined DFNB7 interval. The gene(s) responsible for ARNSHL at these loci resides within an approximately 1 cM interval bounded by markers D9S1806 (centromeric) and D9S769 (telomeric). A recently discovered Indian family confirms the new telomeric boundary. To assist in the identification and cloning of candidate genes, YAC and PAC contigs were constructed. A total of 19 YAC and 23 PAC clones were utilized to span the affected region and ensure double coverage throughout. Twenty-two previously published STSs and 21 new STSs were used to determine marker order and confirm the integrity of the contig. Using a positional cloning strategy we have identified three cochlear expressed genes that map to the DFNB7/11 interval.

Gene Expression

Genetic Markers

Polymerase Chain Reaction

Chromosomes, Artificial, Yeast

Humans

Genotype

Male

Chromosome Mapping

Hearing Loss, Bilateral - congenital

Hearing Loss, Bilateral - genetics

Sequence Analysis, DNA

Chromosomes, Human, Pair 9 - ultrastructure

Homozygote

Pedigree

Female

Consanguinity

Sequence Tagged Sites

Details

Title: Subtitle: Construction of P1-derived artificial chromosome and yeast artificial chromosome contigs encompassing the DFNB7 and DFNB11 region of chromosome 9q13-21
Creators: John H Greinwald Jr - Department of Otolaryngology-Head and Neck Surgery, University of Iowa Hospitals and Clinics, Iowa City 52242, USA
Daryl A Scott
Jacquie R Marietta
Rivka Carmi - University of Iowa
Jose Manaligod
Arabandi Ramesh
Ross I S Zbar
Michelle L Kraft
Khalil Elbedour
Yael Yairi - Soroka Medical Center
Maurice Musy - The University of Texas Southwestern Medical Center
Anne B Skvorak - Brigham and Women's Hospital
Guy Van Camp
C R Srikumari Srisailapathy
Michael Lovett - The University of Texas Southwestern Medical Center
Cynthia C Morton - Brigham and Women's Hospital
Val C Sheffield
Richard J H Smith
Resource Type: Journal article
Publication Details: Genome research, Vol.7(9), pp.879-886
Publisher: United States
DOI: 10.1101/gr.7.9.879
PMID: 9314493
ISSN: 1088-9051
eISSN: 1549-5469
Grant note: HG00457 / NHGRI NIH HHS P50HG00835 / NHGRI NIH HHS R01DCO2842 / NIDCD NIH HHS
Language: English
Date published: 09/1997
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics; Otolaryngology; Internal Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9984006442302771

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