Journal article
Construction of Tn4001 lac derivatives to be used as promoter probe vectors in mycoplasmas
Gene, Vol.137(2), pp.217-222
12/31/1993
DOI: 10.1016/0378-1119(93)90009-R
Abstract
Studies of gene expression in mycoplasmas has been difficult, because the elements involved in gene expression remain relatively undefined. In order to be able to examine these regulatory elements in vivo, derivatives of Tn4001 containing the promoterless
Escherichia coli lacZ reporter gene have been constructed. A Tn4001
lac derivative, Tn4001.2062.2
lac, transforms
Mycoplasma gallisepticum and
Acholeplasma strain ISM1499. Approximately 3% of the
M. gallisepticum and 8% of the
Acholeplasma ISM 1499 transformants appeared to generate
lacZ fusions based on blue colony formation on XGal-containing media. However, placement of
lacZ into an IS256 arm of Tn4001 resulted in a derivative that transformed at a frequency about 3–7-fold lower than that of wild-type Tn4001. Another Tn4001
lac derivative, Tn4007.2065, possesses a plasmid, as well as
lacZ, in the IS256 arm. This construct was designed to permit the direct rescue of adjacent chromosomal sequences that are driving the expression of
lacZ contained within the transposon. These constructs should be useful in locating and defining the upstream elements involved in mycoplasma gene expression.
Details
- Title: Subtitle
- Construction of Tn4001 lac derivatives to be used as promoter probe vectors in mycoplasmas
- Creators
- Kevin L. Knudtson - Iowa State UniversityF.Chris Minion - Iowa State University
- Resource Type
- Journal article
- Publication Details
- Gene, Vol.137(2), pp.217-222
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0378-1119(93)90009-R
- ISSN
- 0378-1119
- eISSN
- 1879-0038
- Language
- English
- Date published
- 12/31/1993
- Academic Unit
- Iowa Institute of Human Genetics
- Record Identifier
- 9984627292502771
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