Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1

Radha Ayyagari; Ann Nestorowicz; Yan Li; Settara Chandrasekharappa; Craig Chinault; Peter van Tuinen; Richard J H Smith; J Fielding Hejtmancik; M Alan Permutt

doi:10.1101/gr.6.6.504

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Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1

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Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1

Radha Ayyagari, Ann Nestorowicz, Yan Li, Settara Chandrasekharappa, Craig Chinault, Peter van Tuinen, Richard J H Smith, J Fielding Hejtmancik and M Alan Permutt

Genome research, Vol.6(6), pp.504-514

06/1996

DOI: 10.1101/gr.6.6.504

PMID: 8828039

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Abstract

The Usher syndrome type 1C (USH1C) and familial hyperinsulinism (HI) loci have been assigned to chromosome 11p14-15.1, within the interval D11S419-D11S1310. We have constructed a yeast artificial chromosome (YAC) contig, extending from D11S926 to D11S899, which encompasses the critical regions for both USH1C and HI and spans an estimated genetic distance of approximately 4 cM. A minimal set of six YAC clones constitute the contig, with another 22 YACs confirming the order of sequence-tagged sites (STSs) and position of YACs on the contig. A total of 40 STSs, including 10 new STSs generated from YAC insert-end sequences and inter-Alu PCR products, were used to order the clones within the contig. This physical map provides a resource for identification of gene transcripts associated with USH1C, HI, and other genetic disorders that map to the D11S926-D11S899 interval.

Genetic Markers - genetics

Humans

Cloning, Molecular

Genetic Diseases, Inborn - genetics

Hyperinsulinism - genetics

Chromosome Mapping

Chromosomes, Human, Pair 11 - genetics

DNA Primers - chemistry

Sequence Tagged Sites

Chromosomes, Artificial, Yeast - genetics

Details

Title: Subtitle: Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1
Creators: Radha Ayyagari - National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Ann Nestorowicz
Yan Li
Settara Chandrasekharappa
Craig Chinault
Peter van Tuinen
Richard J H Smith
J Fielding Hejtmancik
M Alan Permutt
Resource Type: Journal article
Publication Details: Genome research, Vol.6(6), pp.504-514
DOI: 10.1101/gr.6.6.504
PMID: 8828039
NLM abbreviation: Genome Res
ISSN: 1088-9051
eISSN: 1549-5469
Publisher: United States
Grant note: R01-DC02046 / NIDCD NIH HHS DK16746 / NIDDK NIH HHS
Language: English
Date published: 06/1996
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Otolaryngology; Internal Medicine
Record Identifier: 9984007182602771

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