Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection

Bradley D Jones; C Virginia Lockatell; David E Johnson; John W Warren; Harry L T Mobley

doi:10.1128/iai.58.4.1120-1123.1990

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Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection

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Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection

Bradley D Jones, C Virginia Lockatell, David E Johnson, John W Warren and Harry L T Mobley

Infection and immunity, Vol.58(4), pp.1120-1123

04/1990

DOI: 10.1128/iai.58.4.1120-1123.1990

PMCID: PMC258592

PMID: 2180821

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Abstract

Proteus mirabilis, a urease-producing uropathogen, causes serious urinary tract infections in humans. To specifically evaluate the contribution of urease to virulence, a mutation was introduced into P. mirabilis HI4320 by homologous recombination. Virulence was assessed in the CBA mouse model of ascending urinary tract infection. Twenty mice each were challenged transurethrally with P. mirabilis HI4320 and its urease-negative derivative (1 x 10(9) to 2 x 10(9) CFU). At 48 h animals were sacrificed and the mean log10 CFU per milliliter of urine (parent, 6.23; mutant, 4.19; P = 0.0014) or per gram of bladder (parent, 6.29; mutant, 4.28; P = 0.0002), left kidney (parent, 4.11; mutant, 1.02; P = 0.00009), and right kidney (parent, 4.11; mutant, 2.43; P = 0.036) were all shown to be significantly different. These data demonstrate a role for urease as a critical virulence determinant for uropathogenic P. mirabilis.

Details

Title: Subtitle: Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection
Creators: Bradley D Jones - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
C Virginia Lockatell - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
David E Johnson - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
John W Warren - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
Harry L T Mobley - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.58(4), pp.1120-1123
DOI: 10.1128/iai.58.4.1120-1123.1990
PMID: 2180821
PMCID: PMC258592
NLM abbreviation: Infect Immun
ISSN: 0019-9567
eISSN: 1098-5522
Language: English
Date published: 04/1990
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083212802771

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