Journal article
Contact-dependent regulation of N-type calcium channel subunits during synaptogenesis
Journal of neurobiology, Vol.35(2), pp.198-208
05/1998
DOI: 10.1002/(SICI)1097-4695(199805)35:2<198::AID-NEU6>3.0.CO;2-#
PMID: 9581974
Abstract
The developmental regulation of the N-type calcium channel during synaptogenesis was studied using cultured rat hippocampal neurons to elucidate the roles of extrinsic versus intrinsic cues in the expression and distribution of this channel. Prior to synapse formation, alpha1B and beta3 subunits of the N-type calcium channel were distributed diffusely throughout neurites, growth cones, and somata. As synaptogenesis proceeded, the subunit distributions became punctate and colocalized with the synaptic vesicle protein synaptotagmin. Isolated neurons were also examined to test for the requirement of extrinsic cues that control N-type calcium channel expression and distribution. These neurons expressed N-type calcium channel subunits, but their distributions remained diffuse. Functional omega-conotoxin GVIA-sensitive channels were expressed in isolated neurons, although the distribution of alpha1B subunits was diffuse. The distribution of the alpha1B subunit and synaptotagmin only became punctate when neuron-neuron contact was allowed. Thus, the expression of functional N-type calcium channels is the result of an intrinsic program while extrinsic regulatory cues mediated by neuron-neuron contact are required to control their distribution during synaptogenesis.
Details
- Title: Subtitle
- Contact-dependent regulation of N-type calcium channel subunits during synaptogenesis
- Creators
- Fredrick H BahlsRaj LartiusLouis‐Eric TrudeauRobert T DoyleYu FangDerrick WitcherKevin CampbellPhilip G Haydon
- Resource Type
- Journal article
- Publication Details
- Journal of neurobiology, Vol.35(2), pp.198-208
- DOI
- 10.1002/(SICI)1097-4695(199805)35:2<198::AID-NEU6>3.0.CO;2-#
- PMID
- 9581974
- NLM abbreviation
- J Neurobiol
- ISSN
- 0022-3034
- eISSN
- 1097-4695
- Publisher
- John Wiley & Sons, Inc; New York
- Number of pages
- 11
- Grant note
- NIH (NS24233; T32‐A1‐7343) Human Frontiers NSF (BIR‐9113595)
- Language
- English
- Date published
- 05/1998
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068378802771
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