Journal article
Contextual fear conditioning induces differential alternative splicing
Neurobiology of learning and memory, Vol.134(Pt B), pp.221-235
10/2016
DOI: 10.1016/j.nlm.2016.07.018
PMCID: PMC5028328
PMID: 27451143
Abstract
•A number of genes show alternative splicing during learning.•Homer1 isoform Ania-3 is regulated by fear conditioning.•Differential isoform usage can vary with shock only, context only, or fear conditioning.
The process of memory consolidation requires transcription and translation to form long-term memories. Significant effort has been dedicated to understanding changes in hippocampal gene expression after contextual fear conditioning. However, alternative splicing by differential transcript regulation during this time period has received less attention. Here, we use RNA-seq to determine exon-level changes in expression after contextual fear conditioning and retrieval. Our work reveals that a short variant of Homer1, Ania-3, is regulated by contextual fear conditioning. The ribosome biogenesis regulator Las1l, small nucleolar RNA Snord14e, and the RNA-binding protein Rbm3 also change specific transcript usage after fear conditioning. The changes in Ania-3 and Las1l are specific to either the new context or the context-shock association, while the changes in Rbm3 occur after context or shock only. Our analysis revealed novel transcript regulation of previously undetected changes after learning, revealing the importance of high throughput sequencing approaches in the study of gene expression changes after learning.
Details
- Title: Subtitle
- Contextual fear conditioning induces differential alternative splicing
- Creators
- Shane G Poplawski - Pharmacology Graduate Group, University of Pennsylvania, Philadelphia, PA, USALucia Peixoto - Department of Biology, University of Pennsylvania, Philadelphia, PA, USAGiulia S Porcari - Department of Biology, University of Pennsylvania, Philadelphia, PA, USAMathieu E Wimmer - Department of Psychiatry, University of Pennsylvania, Philadelphia, PA, USAAnna G McNally - Pharmacology Graduate Group, University of Pennsylvania, Philadelphia, PA, USAKeiko Mizuno - Centre for the Cellular Basis of Behaviour, King’s College London, London, UKK. Peter Giese - Centre for the Cellular Basis of Behaviour, King’s College London, London, UKSnehajyoti Chatterjee - Department of Biology, University of Pennsylvania, Philadelphia, PA, USAJohn N Koberstein - Elson S. Floyd College of Medicine, Washington State University, Spokane, WA, USADavide Risso - Division of Biostatistics, School of Public Health, University of California, Berkeley, CA, USATerence P Speed - Department of Statistics, University of California, Berkeley, CA, USATed Abel - Pharmacology Graduate Group, University of Pennsylvania, Philadelphia, PA, USA
- Resource Type
- Journal article
- Publication Details
- Neurobiology of learning and memory, Vol.134(Pt B), pp.221-235
- DOI
- 10.1016/j.nlm.2016.07.018
- PMID
- 27451143
- PMCID
- PMC5028328
- NLM abbreviation
- Neurobiol Learn Mem
- ISSN
- 1074-7427
- eISSN
- 1095-9564
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100000002, name: NIH, award: R01MH087463
- Language
- English
- Date published
- 10/2016
- Academic Unit
- Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984065836902771
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