Journal article
Continuous oxygen monitoring of mammalian cell growth on space shuttle mission STS-93 with a novel radioluminescent oxygen sensor
Applied biochemistry and biotechnology, Vol.104(1), pp.1-11
2003
DOI: 10.1385/ABAB:104:1:1
PMID: 12495201
Abstract
A compact, flow-through oxygen sensor device based on luminescence quenching was used to monitor dissolved oxygen levels during mammalian cell growth on the STS-93 mission of the Columbia space shuttle. Excitation of an oxygen-sensitive ruthenium complex was provided by a radioluminescent light source (0.9 mm in diameter, 2.5 mm long), and the intensity of the resulting luminescence was measured by a simple photodiode detector. The use of radioluminescence for the excitation light source is a unique approach that provides many features important for long-term and remote monitoring applications. For the spaceflight experiment, human lung fibroblast cells (WI-38) were grown in hollow-fiber bioreactors. Oxygen concentration was measured in the flow path both before and after the bioreactor cartridge in order to gain information about the metabolism of the cells. The sensor was found to be nonperturbing to cell growth and withstood the challenging physical conditions of shuttle launch and landing while maintaining a stable calibration function. In addition, the sensor provided physically meaningful oxygen predictions.
Details
- Title: Subtitle
- Continuous oxygen monitoring of mammalian cell growth on space shuttle mission STS-93 with a novel radioluminescent oxygen sensor
- Creators
- Julie S Reece - Department of Chemistry, 230 IATL, The University of Iowa, Iowa City, IA 52242, United StatesMichael J Miller - Department of Physics and Astronomy, 230 IATL, The University of Iowa, Iowa City, IA 52242, United StatesMark A Arnold - Department of Chemistry, 230 IATL, The University of Iowa, Iowa City, IA 52242, United StatesCris Waterhouse - Department of Resuscitative Medicine, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United StatesTed Delaplaine - Department of Resuscitative Medicine, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United StatesLaura Cohn - Department of Resuscitative Medicine, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United StatesTom Cannon - Department of Resuscitative Medicine, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Resource Type
- Journal article
- Publication Details
- Applied biochemistry and biotechnology, Vol.104(1), pp.1-11
- Publisher
- Springer
- DOI
- 10.1385/ABAB:104:1:1
- PMID
- 12495201
- ISSN
- 0273-2289
- eISSN
- 1559-0291
- Language
- English
- Date published
- 2003
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984216726702771
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