Journal article
Contrasting regulatory effects of organic acids on aerobic vinyl chloride biodegradation in etheneotrophs
Applied microbiology and biotechnology, Vol.106(18), pp.6335-6346
09/03/2022
DOI: 10.1007/s00253-022-12147-y
Abstract
Vinyl chloride (VC) is a common groundwater pollutant generated during anaerobic biodegradation of chlorinated solvents (e.g., trichloroethene (TCE) or tetrachloroethene (PCE)). Aerobic VC biodegradation by etheneotrophs can support anaerobic PCE and TCE bioremediation to achieve complete removal in situ. However, anaerobic bioremediation strategies necessitate biostimulation with electron donors that are fermented in situ, generating organic acids that could influence aerobic VC biodegradation processes. We examined the effect of organic acids (lactate, acetate, propionate, and butyrate) on aerobic VC biodegradation by VC-assimilating etheneotrophs
Mycobacterium
strain JS60 and
Nocardioides
strain JS614. Strain JS60 grew on all organic acids tested, while strain JS614 did not respond to lactate. VC-grown strain JS60 fed VC and one or more organic acids showed carbon catabolite repression (CCR) behavior where VC biodegradation occurred only after organic acids were depleted. In contrast, CCR was not evident in VC-grown strain JS614, which degraded VC and organic acids simultaneously. Acetate-grown JS60 showed similar CCR behavior when fed VC and a single organic acid, except that extended lag periods (5–12 days) occurred before VC oxidation ensued. Acetate-grown JS614 fed VC and either acetate or butyrate displayed 5–8 day lag periods before simultaneous VC and organic acid biodegradation. In contrast, acetate-grown JS614 degraded VC and propionate without a significant lag, suggesting a regulatory link between propionate and VC oxidation in JS614. Different global regulatory mechanisms controlling VC biodegradation in the presence of organic acids in etheneotrophs have implications for developing combined anaerobic–aerobic bioremediation strategies at chlorinated ethene-contaminated sites.
Key points
• With organic acids present, VC utilization was repressed in JS60, but not in JS614
• Strain JS60 grew readily on lactate, while strain JS614 did not
• Propionate alleviated lag periods for VC utilization in acetate-grown JS614
Details
- Title: Subtitle
- Contrasting regulatory effects of organic acids on aerobic vinyl chloride biodegradation in etheneotrophs
- Creators
- Weilun Zhao - University of IowaPatrick M. Richards - University of IowaTimothy E. Mattes - Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa
- Resource Type
- Journal article
- Publication Details
- Applied microbiology and biotechnology, Vol.106(18), pp.6335-6346
- Publisher
- Springer Berlin Heidelberg
- DOI
- 10.1007/s00253-022-12147-y
- ISSN
- 0175-7598
- eISSN
- 1432-0614
- Grant note
- 1802583 / National Science Foundation (US)
- Language
- English
- Date published
- 09/03/2022
- Academic Unit
- Civil and Environmental Engineering
- Record Identifier
- 9984296037402771
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