Contribution of Drosophila DEG/ENaC genes to salt taste

Lei Liu; A Soren Leonard; David G Motto; Margaret A Feller; Margaret P Price; Wayne A Johnson; Michael J Welsh

doi:10.1016/S0896-6273(03)00394-5

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Contribution of Drosophila DEG/ENaC genes to salt taste

Journal article

Open access

Peer reviewed

Contribution of Drosophila DEG/ENaC genes to salt taste

Lei Liu, A Soren Leonard, David G Motto, Margaret A Feller, Margaret P Price, Wayne A Johnson and Michael J Welsh

Neuron (Cambridge, Mass.), Vol.39(1), pp.133-146

07/03/2003

DOI: 10.1016/S0896-6273(03)00394-5

PMID: 12848938

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https://doi.org/10.1016/S0896-6273(03)00394-5View

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Abstract

The ability to detect salt is critical for the survival of terrestrial animals. Based on amiloride-dependent inhibition, the receptors that detect salt have been postulated to be DEG/ENaC channels. We found the Drosophila DEG/ENaC genes Pickpocket11 (ppk11) and Pickpocket19 (ppk19) expressed in the larval taste-sensing terminal organ and in adults on the taste bristles of the labelum, the legs, and the wing margins. When we disrupted PPK11 or PPK19 function, larvae lost their ability to discriminate low concentrations of Na(+) or K(+) from water, and the electrophysiologic responses to low salt concentrations were attenuated. In both larvae and adults, disrupting PPK11 or PPK19 affected the behavioral response to high salt concentrations. In contrast, the response of larvae to sucrose, pH 3, and several odors remained intact. These results indicate that the DEG/ENaC channels PPK11 and PPK19 play a key role in detecting Na(+) and K(+) salts.

Amino Acid Sequence

Salts

Electrophysiology

Molecular Sequence Data

Behavior, Animal - physiology

Neurons, Afferent - physiology

Animals

In Situ Hybridization

Gene Expression Regulation, Developmental

Polymerase Chain Reaction

Amiloride - pharmacology

Behavior, Animal - drug effects

Sodium Channels - genetics

Larva - physiology

Mutation

Taste - genetics

Embryo, Nonmammalian - physiology

Drosophila - genetics

Details

Title: Subtitle: Contribution of Drosophila DEG/ENaC genes to salt taste
Creators: Lei Liu - Howard Hughes Medical Institute and Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City 52242, USA
A Soren Leonard
David G Motto
Margaret A Feller
Margaret P Price
Wayne A Johnson
Michael J Welsh
Resource Type: Journal article
Publication Details: Neuron (Cambridge, Mass.), Vol.39(1), pp.133-146
Publisher: United States
DOI: 10.1016/S0896-6273(03)00394-5
PMID: 12848938
ISSN: 0896-6273
eISSN: 1097-4199
Grant note: HL-29851 / NHLBI NIH HHS HL-14388 / NHLBI NIH HHS DK25295 / NIDDK NIH HHS
Language: English
Date published: 07/03/2003
Academic Unit: Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Neurosurgery; Internal Medicine
Record Identifier: 9984020887502771

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