Contribution of nitric oxide in the contraction-induced rapid vasodilation in young and older adults

Darren P Casey; Branton G Walker; Sushant M Ranadive; Jennifer L Taylor; Michael J Joyner

doi:10.1152/japplphysiol.00446.2013

Back

Contribution of nitric oxide in the contraction-induced rapid vasodilation in young and older adults

Journal article

Open access

Peer reviewed

Contribution of nitric oxide in the contraction-induced rapid vasodilation in young and older adults

Darren P Casey, Branton G Walker, Sushant M Ranadive, Jennifer L Taylor and Michael J Joyner

Journal of applied physiology (1985), Vol.115(4), pp.446-455

08/15/2013

DOI: 10.1152/japplphysiol.00446.2013

PMCID: PMC3742946

PMID: 23788575

Files and links (1)

url

https://doi.org/10.1152/japplphysiol.00446.2013View

Published (Version of record) Open Access

Abstract

We tested the hypothesis that reduced nitric oxide (NO) bioavailability contributes to the attenuated peak and total vasodilation following single-muscle contractions in older adults. Young ( n = 10; 24 ± 2 yr) and older ( n = 10; 67 ± 2 yr) adults performed single forearm contractions at 10, 20, and 40% of maximum during saline infusion (control) and NO synthase (NOS) inhibition via N G -monomethyl- l -arginine. Brachial artery diameters and velocities were measured using Doppler ultrasound and forearm vascular conductance (FVC; in ml·min −1 ·100 mmHg −1 ) was calculated from blood flow (ml/min) and blood pressure (mmHg). Peak and total vasodilator responses [change (Δ) in FVC from baseline] were attenuated in older adults at all intensities ( P < 0.05). NOS inhibition reduced the peak ΔFVC at 10% (88 ± 12 vs. 52 ± 9 ml·min −1 ·100 mmHg −1 ), 20% (125 ± 13 vs. 83 ± 13 ml·min −1 ·100 mmHg −1 ), and 40% (207 ± 26 vs. 133 ± 20 ml·min −1 ·100 mmHg −1 ) in young subjects, ( P < 0.05 for all) and in older adults at 10% (59 ± 5 vs. 47 ± 7 ml·min −1 ·100 mmHg −1 , P < 0.05) and 20% (88 ± 9 vs. 68 ± 9 ml·min −1 ·100 mmHg −1 , P < 0.05), but not 40% (128 ± 12 vs. 105 ± 11 ml·min −1 ·100 mmHg −1 , P = 0.11). The relative (%) reduction in peak ΔFVC due to NOS inhibition was greater in young vs. older adults at 20% (−36 ± 5 vs. −23 ± 5%, P < 0.05) and 40% (−35 ± 6 vs. −16 ± 7%, P < 0.05). The reduction in the total vasodilator response (area under the curve) with NOS inhibition was also greater in young vs. older adults at all intensities. Our data suggest that contraction-induced rapid vasodilation is mediated in part by NO, and that the contribution of NO is greater in young adults.

muscle contraction

nitric oxide

aging

vasodilation

Details

Title: Subtitle: Contribution of nitric oxide in the contraction-induced rapid vasodilation in young and older adults
Creators: Darren P Casey - Department of Physical Therapy and Rehabilitation Science, Carver College of Medicine, University of Iowa, Iowa City, Iowa; and
Branton G Walker - Department of Anesthesiology, Mayo Clinic, Rochester, Minnesota
Sushant M Ranadive - Department of Anesthesiology, Mayo Clinic, Rochester, Minnesota
Jennifer L Taylor - Department of Anesthesiology, Mayo Clinic, Rochester, Minnesota
Michael J Joyner - Department of Anesthesiology, Mayo Clinic, Rochester, Minnesota
Resource Type: Journal article
Publication Details: Journal of applied physiology (1985), Vol.115(4), pp.446-455
DOI: 10.1152/japplphysiol.00446.2013
PMID: 23788575
PMCID: PMC3742946
NLM abbreviation: J Appl Physiol (1985)
ISSN: 8750-7587
eISSN: 1522-1601
Publisher: American Physiological Society; Bethesda, MD
Language: English
Date published: 08/15/2013
Academic Unit: Physical Therapy and Rehabilitation Science; Fraternal Order of Eagles Diabetes Research Center
Record Identifier: 9984046917702771

Metrics

23 Record Views

52 Times Cited - Web of Science