Journal article
Control of dinucleoside polyphosphates by the FHIT-homologous HNT2 gene, adenine biosynthesis and heat shock in Saccharomyces cerevisiae
BMC Molecular Biology, Vol.3(1), pp.7-7
05/20/2002
DOI: 10.1186/1471-2199-3-7
PMID: 12028594
Abstract
BACKGROUND: The FHIT gene is lost early in the development of many tumors. Fhit possesses intrinsic ApppA hydrolase activity though ApppA cleavage is not required for tumor suppression. Because a mutant form of Fhit that is functional in tumor suppression and defective in catalysis binds ApppA well, it was hypothesized that Fhit-substrate complexes are the active, signaling form of Fhit. Which substrates are most important for Fhit signaling remain unknown. RESULTS: Here we demonstrate that dinucleoside polyphosphate levels increase 500-fold to hundreds of micromolar in strains devoid of the Saccharomyces cerevisiae homolog of Fhit, Hnt2. Accumulation of dinucleoside polyphosphates is reversed by re-expression of Hnt2 and is active site-dependent. Dinucleoside polyphosphate levels depend on an intact adenine biosynthetic pathway and time in liquid culture, and are induced by heat shock to greater than 0.1 millimolar even in Hnt2+ cells. CONCLUSIONS: The data indicate that Hnt2 hydrolyzes both ApppN and AppppN in vivo and that, in heat-shocked, adenine prototrophic yeast strains, dinucleoside polyphosphates accumulate to levels in which they may saturate Hnt2.
Details
- Title: Subtitle
- Control of dinucleoside polyphosphates by the FHIT-homologous HNT2 gene, adenine biosynthesis and heat shock in Saccharomyces cerevisiae
- Creators
- Marta Rubio-TexeiraJames VarnumPawel BieganowskiCharles Brenner
- Resource Type
- Journal article
- Publication Details
- BMC Molecular Biology, Vol.3(1), pp.7-7
- Publisher
- BioMed Central Ltd
- DOI
- 10.1186/1471-2199-3-7
- PMID
- 12028594
- ISSN
- 1471-2199
- eISSN
- 1471-2199
- Language
- English
- Date published
- 05/20/2002
- Academic Unit
- Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9983788429702771
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