Journal article
Control of profilin and actin expression in muscle and nonmuscle cells
Cell motility and the cytoskeleton, Vol.24(3), pp.179-188
1993
DOI: 10.1002/cm.970240305
PMID: 8385578
Abstract
Profilin is a small G‐actin binding protein implicated in sequestering actin monomers in vivo. We have quantitated profilin and actin expression in human hepatoma HepG‐2 cells and in two mouse myogenic cell lines, BC3H1 and C2C12, to determine whether the expression of profilin and the expression of nonmuscle isoactin or total actin are co‐regulated. During differentiation of both muscle cell types, profilin and nonmuscle actin expression decrease in a coordinate manner as shown by measurements of steady state mRNA and newly synthesized protein. In human hepatoma HepG‐2 cells, the twofold increase in actin synthesis observed after 24 hours of exposure to cytochalasin D did not result in an increase in profilin synthesis. Thus, profilin and actin expression are not coregulated in all cells. To determine if there is sufficient profilin to sequester a large portion of cellular G‐actin, we measured total profilin and G‐actin levels in the three cell types. In each case, profilin accounted for less than 10% of the total G‐actin on a molar basis. Thus, profilin is not responsible for total G‐actin sequestration in these cells. Finally, using poly‐L‐proline affinity chromatography, we showed that, in the cell types tested, less than 20% of the poly‐L‐proline purified profilin existed as a complex with G‐actin. The profilin in these cells may be interacting with cellular components other than actin. © 1993 Wiley‐Liss, Inc.
Details
- Title: Subtitle
- Control of profilin and actin expression in muscle and nonmuscle cells
- Creators
- Gary BabcockPeter A Rubenstein
- Resource Type
- Journal article
- Publication Details
- Cell motility and the cytoskeleton, Vol.24(3), pp.179-188
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- DOI
- 10.1002/cm.970240305
- PMID
- 8385578
- ISSN
- 0886-1544
- eISSN
- 1097-0169
- Number of pages
- 10
- Language
- English
- Date published
- 1993
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984024516502771
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