Journal article
Controlled Co-delivery of pPDGF-B and pBMP-2 from intraoperatively bioprinted bone constructs improves the repair of calvarial defects in rats
Biomaterials, Vol.281, pp.121333-121333
02/2022
DOI: 10.1016/j.biomaterials.2021.121333
PMCID: PMC8810707
PMID: 34995904
Abstract
Intraoperative bioprinting (IOB), which refers to the bioprinting process performed on a live subject in a surgical setting, has made it feasible to directly deliver gene-activated matrices into craniomaxillofacial (CMF) defect sites. In this study, we demonstrated a novel approach to overcome the current limitations of traditionally fabricated non-viral gene delivery systems through direct IOB of bone constructs into defect sites. We used a controlled co-delivery release of growth factors from a gene-activated matrix (an osteogenic bioink loaded with plasmid-DNAs (pDNA)) to promote bone repair. The controlled co-delivery approach was achieved from the combination of platelet-derived growth factor-B encoded plasmid-DNA (pPDGF-B) and chitosan-nanoparticle encapsulating pDNA encoded with bone morphogenetic protein-2 (CS-NPs(pBMP2)), which facilitated a burst release of pPDGF-B in 10 days, and a sustained release of pBMP-2 for 5 weeks in vitro. The controlled co-delivery approach was tested for its potential to repair critical-sized rat calvarial defects. The controlled-released pDNAs from the intraoperatively bioprinted bone constructs resulted in ∼40% bone tissue formation and ∼90% bone coverage area at 6 weeks compared to ∼10% new bone tissue and ∼25% total bone coverage area in empty defects. The delivery of growth factors incorporated within the intraoperatively bioprinted constructs could pose as an effective way to enhance bone regeneration in patients with cranial injuries in the future.
Details
- Title: Subtitle
- Controlled Co-delivery of pPDGF-B and pBMP-2 from intraoperatively bioprinted bone constructs improves the repair of calvarial defects in rats
- Creators
- Kazim K Moncal - Engineering Science and Mechanics, Pennsylvania State University, University Park, PA, USAR. Seda Tigli Aydın - Department of Biomedical Engineering, Bulent Ecevit University, Incivez, Zonguldak, TurkeyKevin P Godzik - Biomedical Engineering, Pennsylvania State University, University Park, PA, USATimothy M Acri - Division of Pharmaceutics and Translational Therapeutics, Collage of Pharmacy, University of Iowa, IA, USADong N Heo - Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul, Republic of KoreaElias Rizk - Department of Neurosurgery, Penn State University, College of Medicine, Hershey, PA, USAHwabok Wee - Department of Orthopedics and Rehabilitation, Penn State University, College of Medicine, Hershey, PA, USAGregory S Lewis - Engineering Science and Mechanics, Pennsylvania State University, University Park, PA, USAAliasger K Salem - Division of Pharmaceutics and Translational Therapeutics, Collage of Pharmacy, University of Iowa, IA, USAIbrahim T Ozbolat - Engineering Science and Mechanics, Pennsylvania State University, University Park, PA, USA
- Resource Type
- Journal article
- Publication Details
- Biomaterials, Vol.281, pp.121333-121333
- DOI
- 10.1016/j.biomaterials.2021.121333
- PMID
- 34995904
- PMCID
- PMC8810707
- NLM abbreviation
- Biomaterials
- ISSN
- 0142-9612
- eISSN
- 1878-5905
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 02/2022
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9984216680702771
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