Journal article
Controlled drug delivery from 3D printed two-photon polymerized poly(ethylene glycol) dimethacrylate devices
International journal of pharmaceutics, Vol.552(1-2), pp.217-224
12/01/2018
DOI: 10.1016/j.ijpharm.2018.09.065
PMID: 30268853
Abstract
[Display omitted] Controlled drug delivery systems have been utilized to enhance the therapeutic effects of many drugs by delivering drugs in a time-dependent and sustained manner. Here, with the aid of 3D printing technology, drug delivery devices were fabricated and tested using a model drug (fluorophore: rhodamine B). Poly(ethylene glycol) dimethacrylate (PEGDMA) devices were fabricated using a two-photon polymerization (TPP) system and rhodamine B was homogenously entrapped inside the polymer matrix during photopolymerization. These devices were printed with varying porosity and morphology using varying printing parameters such as slicing and hatching distance. The effects of these variables on drug release kinetics were determined by evaluating device fluorescence over the course of one week. These PEGDMA-based structures were then investigated for toxicity and biocompatibility in vitro, where MTS assays were performed using a range of cell types including induced pluripotent stem cells (iPSCs). Overall, tuning the hatching distance, slicing distance, and pore size of the fabricated devices modulated the rhodamine B release profile, in each case presumably due to resulting changes in the motility of the small molecule and its access to structure edges. In general, increased spacing provided higher drug release while smaller spacing resulted in some occlusion, preventing media infiltration and thus resulting in reduced fluorophore release. The devices had no cytotoxic effects on human embryonic kidney cells (HEK293), bone marrow stromal stem cells (BMSCs) or iPSCs. Thus, we have demonstrated the utility of two-photon polymerization to create biocompatible, complex miniature devices with fine details and tunable release of a model drug.
Details
- Title: Subtitle
- Controlled drug delivery from 3D printed two-photon polymerized poly(ethylene glycol) dimethacrylate devices
- Creators
- Anh-Vu Do - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, United StatesKristan S Worthington - Institute for Vision Research, Department of Ophthalmology and Visual Sciences, College of Medicine, The University of Iowa, United StatesBudd A Tucker - Institute for Vision Research, Department of Ophthalmology and Visual Sciences, College of Medicine, The University of Iowa, United StatesAliasger K Salem - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, United States
- Resource Type
- Journal article
- Publication Details
- International journal of pharmaceutics, Vol.552(1-2), pp.217-224
- DOI
- 10.1016/j.ijpharm.2018.09.065
- PMID
- 30268853
- NLM abbreviation
- Int J Pharm
- ISSN
- 0378-5173
- eISSN
- 1873-3476
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: 1 R01 024605-01, 1 DP2 OD007483-01, 5P30CA086862
- Language
- English
- Date published
- 12/01/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Ophthalmology and Visual Sciences
- Record Identifier
- 9983985815902771
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