Journal article
Controlling transgene expression to study Xenopus laevis metamorphosis
Proceedings of the National Academy of Sciences - PNAS, Vol.101(14), pp.4839-4842
03/26/2004
DOI: 10.1073/pnas.0401011101
PMCID: PMC387335
PMID: 15047886
Abstract
Sperm-mediated transgenesis of
Xenopus laevis
is the first application of genetic methodology to an amphibian. However, some transgenes are lethal when they are expressed constitutively. To study the influence of these genes on amphibian metamorphosis and to generate F
1
progeny from mature transgenic adults, these transgenes must be placed under the control of an inducible system so that they can be activated at specific times in development. We show that two well known binary inducible gene expression systems supplement transgenesis for the study of
X. laevis
metamorphosis, one system controlled by the progesterone analogue RU-486 and the other controlled by the tetracycline derivative doxycycline. By inducing a dominant negative form of the thyroid hormone receptor under the control of doxycycline specifically in the nervous system we have delimited the developmental periods within which thyroid hormone controls innervation of the developing limb from the spinal cord.
Details
- Title: Subtitle
- Controlling transgene expression to study Xenopus laevis metamorphosis
- Creators
- Biswajit Das - Carnegie Institution for ScienceDonald D. Brown - Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.101(14), pp.4839-4842
- Publisher
- National Academy of Sciences
- DOI
- 10.1073/pnas.0401011101
- PMID
- 15047886
- PMCID
- PMC387335
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Language
- English
- Date published
- 03/26/2004
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984361572602771
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